Diagenode

KAP1 polyclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410236-25
25 μg
$125.00
  Bulk order
Other format

Alternative names: KAP-1, TRIM28, KRIP-1, RNF96, TIF1B, TF1B, TIF1beta

Polyclonal antibody raised in rabbit against KAP1 (tripartite motif containing 28), using a recombinant protein.

Lot39911
Concentration1 μg/μl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2 μg/ChIP Fig 1, 2
Western Blotting 1:1,000 - 1:10,000 Fig 3, 4
Immunoprecipitation 2 μg per IP Fig 5
Immunofluorescence 1:100-1:1,000 Fig 6
Immunohistochemistry 1:100-1:1,000 Fig 7

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    Figure 1. ChIP results obtained with the Diagenode antibody directed against KAP1
    ChIP assays were performed using HeLa cells, the Diagenode antibody against KAP1 (Cat. No. C15410236) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers located in the ZNF180 and L1TD1 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAP1
    ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against KAP1 (Cat. No. C15410236) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 Kb region of human chromosome 12 (fig 2A and B), and in a two genomic regions surrounding the ZNF180 and L1TD1 positive control genes.

    Figure 3. Western blot analysis using the Diagenode antibody directed against KAP1
    Whole cell extracts (30 μg) from H1229 (lane 1) and HeLa cells (lane 2) were analysed by Western blot using the Diagenode antibody against KAP1 (Cat. No. C15410236) diluted 1:10,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Figure 4. Western blot analysis using the Diagenode antibody directed against KAP1
    Whole cell extracts (30 μg) from NIH3T3 cells were analysed by Western blot using the Diagenode antibody against KAP1 (Cat. No. C15410236) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Figure 5. Immunoprecipitation using the Diagenode antibody directed against KAP1
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against KAP1 (Cat. No. C15410236). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated KAP1 protein was detected by western blot with the KAP1 antibody diluted 1:1,000. The IP with the KAP1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (50 μg of HeLa whole cell extract).

    Figure 6. Immunofluorescence with the Diagenode antibody directed against KAP1
    A431 cells were fixed with formaldehyde and stained with the Diagenode antibody against KAP1 (Cat. C15410236) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain.

    Figure 7. Immunohistochemistry using the Diagenode antibody directed against KAP1
    Formalin fixed paraffin embedded breast carcinoma cells were stained with the Diagenode antibody against KAP1 (Cat. No. C15410236) diluted 1:500 followed by a peroxidase labelled goat anti-rabbit secondary antibody.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet KAP1 C15410236 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

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