Diagenode

Hydroxymethyl dCTP

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Catalog Number
Format
Price
C11000002
(AF-102-0300)
250 µl
$495.00

Hydroxymethyl dCTP can be used as a substrate for MethylTaq DNA polymerase (Cat. No. AF-103-0250) permitting the amplification of DNA containing hydroxymethylated cytosine in place of cytosine.

  • Characteristics

    Full name: 5-hydroxymethyl-2'-deoxycytidine-5'-triphosphate (Lithium Salt)

    Formula: C10H14Li4N3O14P3

    Molecular Weight: 520.9 g/mol

    Lambda max: 275 nm

    Description

    • hmdCTP is supplied as 100 mM aqueous solution titrated to pH 7.5
    • > 99% pure by spectral and HPLC analysis
    • hmdCTP can be enzymatically incorporated into DNA with MethylTaq DNA polymerase (Cat. No. AF-103-0250)
    • Hyrdoxymethylated substrates can be ligated by standard ligases
    • Free of endo-, exodeoxyribonuclease, phosphatise and nicking activities
  • Applications
    Methylated DNA immunoprecipitation
    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is ... Read more
  • Documents
    Datasheet Hydroxymethyl dCTP AF-102 DATASHEET
    Datasheet description
    Download
  • Publications

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