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<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
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<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
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<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
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<p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p>
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<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
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<option value="SI">Slovenia</option>
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<option value="SO">Somalia</option>
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<option value="GS">South Georgia and the South Sandwich Islands</option>
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<option value="SJ">Svalbard and Jan Mayen</option>
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<option value="TO">Tonga</option>
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<option value="TC">Turks and Caicos Islands</option>
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<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->
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<h6 style="height:60px">Megaruptor® 2</h6>
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<h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6>
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<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
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<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
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<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
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<a href="/en/p/megaruptor2-1-unit"><img src="/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt=" DNA Shearing, RNA shearing and Chromatin shearing" class="th"/></a> </div>
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<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B06010002</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2829" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 2</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
</div>
</div>
<form action="/en/quotes/quote?id=2829" id="Quote-2829" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2829" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="VHpLM01PY2RsKy80U1h2MG13Qmc5QT09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="megaruptor2-1-unit" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>
<div class="row collapse">
<h2>Contact Information</h2>
<div class="small-3 large-2 columns">
<span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Phone number</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">City</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2829">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d'Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People's Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People's Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
$('#country_selector_quote-2829').selectize();
</script><br />
</div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">State</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][state]" id="state-2829" maxlength="3" type="text"/><br />
</div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div>
</div>
<div class="row collapse" id="email_v">
<div class="small-3 large-2 columns">
<span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Comment</span>
</div>
<div class="small-9 large-10 columns">
<textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea> </div>
</div>
<!------------SERVICES PARTICULAR FORM START---------------->
<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->
<div class="row collapse">
<div class="small-3 large-2 columns">
</div>
<div class="small-9 large-10 columns">
<div class="recaptcha"><div id="recaptcha66224cddd4971"></div></div> </div>
</div>
<br />
<div class="row collapse">
<div class="small-3 large-2 columns">
</div>
<div class="small-9 large-10 columns">
<button id="submit_btn-2829" class="alert button expand" form="Quote-2829" type="submit">Contact me</button> </div>
</div>
</form><script>
var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';
function postToPardot(formAction, id) {
$('#pardot-form-handler').load(function(){
$('#Quote-' + id).attr('action', formAction);
$('#Quote-' + id).submit();
});
$('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
}
$(document).ready(function() {
$('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
$('#Quote-2829').attr('action','javascript:postToPardot(\'' + $('#Quote-2829').attr('action') + '\', 2829)');
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$("#Quote-2829 #submit_btn-2829").click(function (e) {
if($(this).closest('form')[0].checkValidity()){
e.preventDefault();
//disable the submit button
$("#Quote-2829 #submit_btn-2829").attr("disabled", true);
$("#Quote-2829 #submit_btn-2829").html("Processing...");
//submit the form
$("#Quote-2829").submit();
}
})
</script>
<script>
if ($("#Quote-2829 #country_selector_quote-2829.selectized").val() == 'US') {
var val = $("#state-2829").val();
$("#Quote-2829 #state-2829").replaceWith('<select name="data[Quote][state]" id="state-2829" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
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var val = $("#state-2829").val();
$("#Quote-2829 #state-2829").replaceWith('<select name="data[Quote][state]" id="state-2829" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
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$("#Quote-2829 #state-2829").replaceWith('<select name="data[Quote][state]" id="state-2829" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
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<h6 style="height:60px">Megaruptor® 2</h6>
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<h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6>
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<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
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<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
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<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
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<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
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<h6 style="height:60px">Megaruptor® 2</h6>
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'description' => '<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>
<p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>',
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'label2' => 'Demonstrated shearing to fragment sizes between 10 kb and 75 kb with Megaruptor® 2',
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<h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6>
<h6 style="text-align: center;"><em>* indicates unsheared DNA</em></h6>',
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'catalog_number' => 'B06010002',
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'price_EUR' => '17500',
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'modified' => '2021-05-28 10:49:08',
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<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
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<p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p>
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<h6 style="height:60px">Megaruptor® 2</h6>
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<p>The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from <strong>3 kb - 75 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>',
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<h6 style="text-align: center;">Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. <strong>A:</strong> DNA was analyzed by pulsedfield gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software. <strong>B:</strong> Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 10, 15, 20, 30 and 40 kb. (High Sensitivity Large Fragment Analysis Kit; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).</h6>
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<p><span>The Megaruptor</span><span>® </span><span>was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA in the range of 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. </span></p>
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×
