Diagenode

Huntingtin monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200226
50 μg/ 50 μl
$295.00
  Bulk order

Monoclonal antibody raised in mouse against Huntigntin, using a recombinant protein. 

Lot001
Concentration1 μg/μl
Species reactivityHuman, mouse
TypeMonoclonal
PurityAffinity purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunofluorescence 1:400 Fig 2
  • Validation data

    WB

    Western blot analysis using the Diagenode monoclonal antibody directed against Huntingtin
    Mouse brain lysates were analysed by Western blot using the Diagenode antibody against Huntingtin (Cat. No. C15200226) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left

    IF

    Immunofluorescence using the Diagenode monoclonal antibody directed against Huntingtin
    SKNSH cells were stained with the Diagenode antibody against Huntingtin (Cat. No. C15200226) diluted 1:400 followed by an anti-mouse antibody conjugated to DyLight 488 (green color). WGA conjugated to Alexa Fluor 594 was used to stain the plasma membranes (red). The nuclei were stained with DAPI (blue).

  • Background

    Huntingtin (HTT, UniProt/Swiss-Prot entry P42858) may play a role in microtubule-mediated transport or vesicle function and is required for normal development. It is widely expressed as 2 alternatively polyadenylated transcripts, the larger one being predominantly present in adult and fetal brain. The HTT gene is linked to Huntington’s disease, a neurodegenerative disorder characterized by loss of striatal neurons, which is thought to be caused by an expanded, unstable trinucleotide repeat. Whether this leads to loss of function or gain of function is not clear. The expanded repeat may increase the affinity of the HTT protein for the huntingtin-associated protein-1, which is highly expressed in brain and seems to inhibit HTT expression. 

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    Datasheet C15200226 Huntingtin monoclonal antibody DATASHEET
    Huntingtin monoclonal antibody - Classic
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Huntingtin monoclonal antibody - Classic (Diagenode Cat# C15200226 Lot# 001). Click here to copy to clipboard.

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    Oct 3-Oct 8, 2016
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    Oct 3-Oct 4, 2016
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