Diagenode

HP1α polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410070
(pAb-070-050)
50 µg/25 µl
$295.00
  Bulk order

Alternative names: HP1 alpha, CBX5, HP1A

Polyclonal antibody raised in rabbit against human HP1α (Heterochromatin protein 1 homolog alpha), using the full length recombinant GST tagged protein.

Lot001
Concentration2.0 µg/µl
Species reactivityHuman
TypePolyclonal
PurityProtein G purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunofluorescence 1:500 Fig 2
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
  • Validation Data

    Western blot

    Figure 1. Western blot analysis using the Diagenode antibody directed against HP1α
    Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Diagenode antibody against human HP1α (Cat. No. C15410070), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right. The western blot analysis clearly demonstrates that the HP1α antibody specifically recognizes HP1α and not HP1β and HP1γ.

    Immunofluorescence

    Figure 2. Immunofluorescence using the Diagenode antibody directed against HP1α
    HeLa cells were stained with the Diagenode antibody against HP1α (Cat. No. C15410070) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    Datasheet hHP1alpha pAb-070-050 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HP1α polyclonal antibody - Classic (Diagenode Cat# C15410070 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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    Crete, Greece
    Oct 3-Oct 8, 2016
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