Diagenode

His tag monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200191
(MAb-191-050)
50 μg/24 μl
$295.00
  Bulk order

Monoclonal antibody raised in mouse against the 6xHis tag (amino acid sequence HHHHHH) using a KLH-conjugated synthetic peptide. The antibody recognizes the Histidine epitope which is one of the most popular protein tags. The Diagenode His tag antibody is extremely specific and allows unambiguous identification and quantitative analysis of His-tagged proteins.

Lot001
Concentration2.1 µg/µl
Species reactivitySpecific for His-tagged fusion proteins expressed in bacteria, insect, mammalian cells
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution* References
Western Blotting 1:1,000 - 1:3,000 Fig 1, 2
IP/ChIP 1:150 - 1:200
IS 1:500 - 1:1,000

* Please note that the optimal antibody amount should be determined by the end-user for each specific assay condition.

  • Validation Data

    Western Blot

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag
    Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody.

    Western Blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag
    Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet HisTag MAb-191-050 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: His tag monoclonal antibody - Classic (Diagenode Cat# C15200191 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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