Diagenode

HIF1 alpha polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410234-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against HIF1 alpha (hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)), using a recombinant protein.

Lot41185
Concentration1 μg/μl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Figure 1
ICC/IF 1:100 - 1:1,000/td> Figure 7
Immunohistochemistry 1:100 - 1:1,000 Figure 3
Immunoprecipitation 1:100 - 1:500 Figure 2
Western Blotting 1:1,000 - 1:10,000 Figure 4, 5, 6
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    HIF1 alpha ChIP results

    Figure 1. HIF1 alpha ChIP results
    HIF1 alpha antibody immunoprecipitates HIF1 alpha protein-DNA complex in ChIP experiments. ChIP Sample: HepG2 whole chromatin extract under hypoxia (1%O2) treatment 24hr A: 5 μg preimmune rabbit IgG. B: 5 μg of HIF1 alpha antibody The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.

    HIF1 alpha IP results

    Figure 2. HIF1 alpha IP results
    HIF1 alpha antibody immunoprecipitates HIF1 alpha protein in IP experiments. IP Sample: 950 μg whole cell extract of HIF1 alpha protein expressing HeLa cells. A. 20 μg whole cell extract of transfected HeLa cells B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HIF1 alpha protein by 2.5 μg of HIF1 alpha antibody. The immunoprecipitated HIF1 alpha protein was detected by western blot with the HIF1 alpha antibody diluted 1:10,000.

    HIF1 alpha IHC results

    Figure 3. HIF1 alpha IHC results
    HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis. Sample: Paraffin-embedded human kidney cancer. HIF1 alpha antibody dilution: 1:500.

    HIF1 alpha Western blot

    Figure 4. HIF1 alpha Western blot results
    HIF1 alpha antibody detects HIF1 alpha protein in CoCl2-treated samples by Wesern blot analysis. A: 30 μg of HeLa cell lysate (normoxia) B: 30 μg of HeLa cell lysate (500 μM CoCl2 treatment for 24 hrs) 7.5% SDS-PAGE HIF1 alpha antibody dilution: 1:1,000

    HIF1 alpha Western blot

    Figure 5. HIF1 alpha Western blot results
    HIF1 alpha antibody detects HIF1 alpha protein in CoCl2-treated samples by Western blot analysis. A. 30 μg NIH-3T3 whole cell extract (untreated) B. 30 μg NIH-3T3 whole cell extract (200 μM CoCl2 treatment for 24hr) HIF1 alpha antibody dilution: 1:1,000

    HIF1 alpha Western blot

    Figure 6. HIF1 alpha Western blot results
    HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. A. 30 ug HepG2 whole cell extract (untreated) B. 30 μg HepG2 whole cell extract ( 200 μM CoCl2 treatment for 24 hr) C. 30 μg HepG2 whole cell extract (500 μM CoCl2 treatment for 24 hr) 7.5 % SDS-PAGE HIF1 alpha antibody dilution: 1:5000

    HIF1 alpha IF

    Figure 7. HIF1 alpha IF results
    HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by immunofluorescent analysis. Samples: Untreated HeLa cells (left panel) and Hela cells treated with 500 μM CoCl2 for 24 hrs (right panel) were fixed in 4% paraformaldehyde for 15 min. Green: HIF1alpha protein stained by HIF1 alpha antibody diluted 1:1,000 Red: Alpha-tubulin, a cytoskeleton marker.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet HIF1 alpha C15410234 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

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