1ml frozen cell vial containing 1 x 106 cells
Cell Line validation
1ml frozen cell vial containing 1 x 106 cells. Expression of histone H2A is confirmed by immunodetection and confocal microscopy. Stable expression over multiple cell passages is confirmed. The doubling time for the cell line was approximately 20 h for the linear section of the growth curve.
Left panel corresponds to immunodetection of H2A with the anti-HA antibody.
Right panel shows the Dapi DNA staining.
Mycoplasma negative by DNA staining and direct culture methods.