HDAC3 monoclonal antibody - Classic

Catalog Number
100 µl
  Bulk order

Alternative names: HD3, RPD3, RPD3-2, SMAP45

Monoclonal antibody raised in mouse against human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.

Concentrationnot determined
Species reactivityHuman
PurityConcentrated supernatant
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μl/ChIP Fig 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.

  • Validation Data


    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against HDAC3
    ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against HDAC3 (Cat. No. C15100145 ) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (Cat. No. C01010070), on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. Respectively, 5 μl of the HDAC3 monoclonal antibody and 5 μg of IgG (negative IP control) were used. QPCR was performed with primers for the promoters of the active genes c-fos (cat. No. pp-1004-050) and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  • Applications
  • Documents
    Datasheet HDAC3 SN-145-100 DATASHEET
    Datasheet description
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HDAC3 monoclonal antibody - Classic (Diagenode Cat# C15100145 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  • Related products


 See all events

Twitter feed


 See all news