Diagenode

H4K8ac polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410235-25
25 μg
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against Histone H4 (acetyl Lys8) (histone cluster 1, H4a), using a KLH-conjugated synthetic peptide.

Lot41423
Concentration1 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Figure 1
Dot Blotting 1:5,000 Figure 3
ICC/IF 1:100 - 1:1,000 Figure 5
IHC 1:100 - 1:1,000 Figure 6, 7, 8
Immunoprecipitation 1:100 - 1:5,000 Figure 4
Western Blotting 1:1,000 - 1:10,000 Figure 2
* Optimal dilutions/concentrations should be determined by the researcher..
  • Validation Data

    ChIP

    Figure 1. H4K8ac
    ChIP results ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or H4K8ac antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.

    WB

    Figure 2. H4K8ac WB results
    H4K8ac antibody detects Histone H4 acetylated at Lys8 by Western blot analysis.
    A. 30 μg HeLa whole extract (0.012% DMSO treatment for 18 hr)
    B. 30 μg HeLa whole extract (0.4 μM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE H4K8ac antibody dilution: 1:5,000

    Dot Blot

    Figure 3. H4K8ac
    Dot blot results Dotblot analysis of H4K8ac antibody with different peptides. Varied amount of the peptides were spotted onto positively charged nylon membrane and blotted with the H4K8ac antibody at a 1:5,000 dilution. A: Peptide H4K8un B: Peptide H4K8ac C: Peptide H4K16un D: Peptide H4K16ac

    immunoprecipitates

    Figure 4. H4K8ac IP results
    H4K8ac antibody immunoprecipitates Histone H4 acetylated at Lys8 in IP experiments. IP Sample: HeLa whole cell extract
    A: 30 μg whole cell extract of HeLa cells (0.4 uM TSA treatment for 18hr)
    B: Control with 2.5 μg of pre-immune rabbit IgG C: Immunoprecipitation of Histone H4 acetylated at Lys8 by 2.5 μg of H4K8ac antibody. The immunoprecipitated Histone H4 (acetyl K8) protein was detected by western blot with the H4K8ac antibody diluted 1:5,000.

    immunofluorescent

    Figure 5. H4K8ac IF results
    H4K8ac antibody detects Histone H4 acetylated at Lys8 in the nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 acetylated at Lys8 stained by H4K8ac antibody diluted 1:500. Red: alpha Tubulin antibody, a cytoskeleton marker. Blue: Hoechst 33342 staining.

    immunohistochemical

    Figure 6. H4K8ac
    IHC results H4K8ac antibody detects Histone H4 acetylated at Lys8 at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. H4K8ac antibody dilution: 1:500.

    immunohistochemical

    Figure 7. H4K8ac
    IHC results H4K8ac antibody detects Histone H4 acetylated at Lys8 at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. H4K8ac antibody dilution: 1:500.

    immunohistochemical

    Figure 8. H4K8ac
    IHC results H4K8ac antibody detects Histone H4 acetylated at Lys8 at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. H4K8ac antibody dilution: 1:500.

  • Applications
    DB
    Dot blotting Read more
    IC
    Immunochemistry Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H4K8ac C15410235 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

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