Diagenode

H4K20me3 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15100148
(SN-148-100)
100 µl
$295.00
  Bulk order

Monoclonal antibody raised in mouse against histone H4 containing the trimethylated lysine 20 (H4K20me3), using a KLH-conjugated synthetic peptide.

Lot001
Concentrationnot determined
Species reactivityHuman, zebrafish
TypeMonoclonal
PurityConcentrated supernatant
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 3 μl/ChIP Fig 1
Western Blotting 1:50 Fig 2
Immunofluorescence 1:200 Fig 3
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.
  • Validation Data

    ChIP

    Figure 1 ChIP results obtained with the Diagenode monoclonal antibody directed against H4K20me3
    ChIP assays were performed using human HeLa cells, the Diagenode monclonal antibody against H4K20me3 (cat. No. SN-148-100) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (cat. No. kch-maglow-016), using sheared chromatin from 10,000 cells. Two different quantities of antibody (3 and 12 μl per ChIP experiment) were analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive gene TSH2B. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Western Blot

    Figure 2 Western blot analysis using the Diagenode monoclonal antibody directed against H4K20me3
    Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode monoclonal antibody against H4K20me3 (cat. No. SN-148-100) diluted 1:50 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunofluorescence

    Figure 3 Immunofluorescence with the Diagenode monoclonal antibody directed against H4K20me3
    HEK293T cells were stained with the Diagenode antibody against H4K20me3 (cat. No. SN-148-100, green), with human autoantibodies against the centromeres (CREST, Cortex Biochem, pink) and with DAPI (blue). Cells were fixed with 4% paraformaldehyde and blocked with PBS containing 0.1% Tween, 2% BSA, and 5% normal goat serum. Cells were immunofluorescently labeled with the H4K20me3 antibody (diluted 1:200 in blocking solution) and the CREST antibody (diluted 1:1000 in blocking solution) by overnight incubation at 4°C. Subsequently cells were labeled with goat-anti-mouse antibody conjugated to Alexa488 and goat-anti-human antibody conjugated to Alexa633 (both diluted 1:200 in blocking solution and incubated for 1 hour at RT). Cells were mounted with Vectashield containing DAPI. Immunofluorescence wwas performed by Dr. E. Baart, Erasmus University, Rotterdam, the Netherlands.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H4K20me3 SN-148-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K20me3 monoclonal antibody - Classic (Diagenode Cat# C15100148 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Role of Annexin gene and its regulation during zebrafish caudal fin regeneration
    Saxena S, Purushothaman S, Meghah V, Bhatti B, Poruri A, Meena Lakshmi MG, Sarath Babu N, Murthy CL, Mandal KK, Kumar A, Idris MM
    The molecular mechanism of epimorphic regeneration is elusive due to its complexity and limitation in mammals. Epigenetic regulatory mechanisms play a crucial role in development and regeneration. This investigation attempted to reveal the role of epigenetic regulatory mechanisms, such as histone H3 and H4 lysine ac...

  • Related products

Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
 See all events

Twitter feed

News

 See all news