Figure 2. Western blot analysis using the Diagenode antibody directed against H3pan
Histone extracts of NB4 (human promyelocytic leukemia) cells, treated with all-trans retinoic acid (ATRA) to induce cell differentiation, were analysed by Western blot using the Diagenode antibody against H3pan (Cat. No. pAb-059-050), diluted of 1:500 in TBS-Tween, containing 5% skimmed milk. Figure 2 shows the results for cells treated with ATRA for 168 h (lane 1) and 24 h (lane 2) and of untreated control cells (lane 3). A molecular weight marker (in kDa) is shown on the left, the location of the protein of interest is shown on the right.