Diagenode

H3pan polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310059
(CS-059-100)
100 µl
$195.00
  Bulk order

Polyclonal antibody raised in rabbit against histone H3, using a KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein.

LotA75-001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:50 Fig 1
Immunofluorescence 1:500 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody against H3pan (Cat. No. CS-059-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:500.

    Immunofluorescence

    Figure 2. Immunofluorescence using the Diagenode antibody directed against H3pan
    HeLA cells were stained with the Diagenode antibody against H3pan (Cat. No. CS-059-100) and with DAPI. Cells were formaldehyde fixed, permeabilized with sodium citrate and Triton X-100 and blocked with PBS containing 2.5% BSA. Figure 4A: cells were immunofluorescently labelled with the H3pan antibody (diluted 1:500 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to DyLight 488. Figure 4B: Staining of the nuclei with DAPI, which specifically labels DNA. Both, antibody and DAPI staining are restricted to the nucleus. The antibody is directed against H3, regardless of the presence of modifications. This explains the uniform nuclear distribution of the signal.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    Datasheet H3pan CS-059-100 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3pan polyclonal antibody - Pioneer (Diagenode Cat# C15310059 Lot# A75-001 ). Click here to copy to clipboard.

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