Figure 2. Immunofluorescence using the Diagenode antibody directed against H3pan
HeLA cells were stained with the Diagenode antibody against H3pan (Cat. No. CS-059-100) and with DAPI. Cells were formaldehyde fixed, permeabilized with sodium citrate and Triton X-100 and blocked with PBS containing 2.5% BSA. Figure 4A: cells were immunofluorescently labelled with the H3pan antibody (diluted 1:500 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to DyLight 488. Figure 4B: Staining of the nuclei with DAPI, which specifically labels DNA. Both, antibody and DAPI staining are restricted to the nucleus. The antibody is directed against H3, regardless of the presence of modifications. This explains the uniform nuclear distribution of the signal.