Figure 1. Cross reactivity tests using the Diagenode antibody directed against H3K4me3
A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K4me3 (cat# CS-030-100) with peptides containing other modifications and unmodified sequences of histone H3. Figure 1A shows the dot blot with mono-, di- and tri-methylated lysine 4 and the unmodified histone H3. Figure 1B shows the dot blot with the trimethylated lysines 4, 9 and 27 of histone H3 and lysine 20 of histone H4. These results show a slight cross reaction with the trimethylated lysine 20 of histone H4.