H3K4me3 polyclonal antibody - Classic

Catalog Number
100 µl
  Bulk order

Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 4 (H3K4me3), using a KLH-conjugated synthetic peptide.

Concentrationnot determined
Species reactivityHuman
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Dot Blotting - Fig 1
Western Blotting 1:500 Fig 2
  • Validation Data

    Dot Blot

    Figure 1. Cross reactivity tests using the Diagenode antibody directed against H3K4me3
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K4me3 (cat# CS-030-100) with peptides containing other modifications and unmodified sequences of histone H3. Figure 1A shows the dot blot with mono-, di- and tri-methylated lysine 4 and the unmodified histone H3. Figure 1B shows the dot blot with the trimethylated lysines 4, 9 and 27 of histone H3 and lysine 20 of histone H4. These results show a slight cross reaction with the trimethylated lysine 20 of histone H4.

    Western Blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against H3K4me3
    Western blot was performed using histone extracts from HeLa cells (HeLa HE, 15 μg) and the Diagenode antibody against H3K4me3 (cat# CS-030-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right.

  • Applications
    Dot blotting Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet H3K4me3 CS-030-100 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K4me3 polyclonal antibody - Classic (Diagenode Cat# C15310030 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The histone methyltransferase Dot1 is required for DNA damage repair and proper development in Dictyostelium.
    Müller-Taubenberger A, Bönisch C, Fürbringer M, Wittek F, Hake SB
    Posttranslational histone modifications play an important role in modulating gene expression and chromatin structure. Here we report the identification of histone H3K79 dimethylation in the simple eukaryote Dictyostelium discoideum. We have deleted the D. discoideum Dot1/KMT4 homologue and demonstrate that it is the...

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