H3K36me2 monoclonal antibody - Classic

Catalog Number
50 μg/50 μl
  Bulk order

Monoclonal antibody raised in mouse against histone H3 dimethylated at lysine 36 (H3K36me2), using a KLH-conjugated synthetic peptide.

Concentration1.0 µg/µl
Species reactivityHuman
PurityProtein A purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-2 μg/ChIP Fig 1
ELISA 1:3,000 Fig 2
Dot Blotting 1:10,000
Western Blotting 1:1,000 - 1:2,000
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
  • Validation Data


    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me2
    ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against H3K36me2 (Cat. No. C15200182) and optimized PCR primer pairs for qPCR. ChIP was performed with the “Auto Histone ChIP-seq” kit (cat. No. C01010020), using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).


    Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against H3K36me2
    To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H3K36me2 (cat. No. MAb-182-050). The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure 1 shows a high specificity of the antibody for the peptide containing the modification of interest.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Dot blotting Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H3K36me2 MAb-182-050 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K36me2 monoclonal antibody - Classic (Diagenode Cat# C15200182 Lot# 001-11). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature.
    Gonzalez I, Munita R, Agirre E, Dittmer TA, Gysling K, Misteli T, Luco RF
    Alternative pre-mRNA splicing is a highly cell type-specific process essential to generating protein diversity. However, the mechanisms responsible for the establishment and maintenance of heritable cell-specific alternative-splicing programs are poorly understood. Recent observations point to a role of histone modi...

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