Diagenode

H3K23ac polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310140
(CS-140-100)
100 µl
$295.00
  Bulk order

Polyclonal antibody raised in rabbit against histone H3 containing the acetylated lysine 23 (H3K23ac), using a KLH-conjugated synthetic peptide.

LotA615-001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:500 Fig 1
Dot Blotting 1:1,000 Fig 2
Western Blotting 1:250 Fig 3
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3K23ac (Cat. No. CS-140-100). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,750.

    ELISA

    Figure 2. Cross reactivity test using the Diagenode antibody directed against H3K23ac
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K23ac (Cat. No. CS-140-100) with with peptides containing other modifications and unmodified sequences of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody against H3K23ac
    HeLa cells were treated with butyrate and histone extracts (15 μg) were analysed by Western blot using the Diagenode antibody against H3K23ac (Cat. No. CS-140-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk (lane 1). Lane 2 shows a coomassie blue staining of the gel. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet H3K23ac CS-140-100 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

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