Diagenode

H3K18me3 Antibody

Catalog Number
Format
Price
C15410292
50 μg
$480.00
  Bulk order



Polyclonal antibody raised in rabbit against Histone H3 (trimethyl Lys18), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.6 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP 2-5 μg/million cells
Immunofluorescence 1:50 Fig 1
Western Blotting 1:500 Fig 2
Immunochemistry 1:50
Dot Blotting 1:1,000 Fig 3
  • Validation Data

    Immunofluorescence

    Figure 1. Immunofluorescence
    Immunofluorescence of H3K18me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K18me3 is nuclear and chromosomal. Staining: Histone H3K18me3 is expressed in green, nuclei are counterstained with DAPI (blue).

    Western Blot

    Figure 2. Western Blot
    Western Blot of H3K18me3 antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot Blot

    Figure 3. Dot Blot
    Dot Blot of H3K18me3 antibody. Lane 1: K18 unmodified. Lane 2: K18Me1. Lane 3: K18Me2. Lane 4: K18Me3. Lane 5: K18Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 0.5 μg/ml for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  •  Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
    IC
    Immunochemistry Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3K18me3 C15410292 DATASHEET
    Datasheet description
    Download
  •  Safety sheets
    SDS C15410292 H3K18me3 Antibody US en Download
    SDS C15410292 H3K18me3 Antibody GB en Download
    SDS C15410292 H3K18me3 Antibody BE fr Download
    SDS C15410292 H3K18me3 Antibody FR fr Download
    SDS C15410292 H3K18me3 Antibody ES es Download
    SDS C15410292 H3K18me3 Antibody DE de Download
    SDS C15410292 H3K18me3 Antibody JP ja Download
    SDS C15410292 H3K18me3 Antibody BE nl Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K18me3 Antibody (Diagenode Cat# C15410292 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  Related products

Events

  • AACR 2024
    San Diego, California, USA
    Apr 5-Apr 10, 2024
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics