H3K18ac polyclonal antibody - Classic

Catalog Number
100 µl
  Bulk order

This antibody has been raised against histone H3 containing the acetylated lysine 18 (H3K18ac), using a KLH-conjugated synthetic peptide.

Concentrationnot determined
Species reactivityHuman
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 Fig 1
Dot Blotting 1:1,000 Fig 2
Western Blotting 1:250 Fig 3
  • Validation Data


    Figure 1. Determination of the antibody titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3K18ac (Cat. No. CS-139-100). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,800.

    Dot Blot

    Figure 2. Cross reactivity test using the Diagenode antibody directed against H3K18ac
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K18ac (Cat. No. CS-139-100) with with peptides containing other modifications and unmodified sequences of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against H3K18ac
    HeLa cells were treated with butyrate and histone extracts (15 μg) were analysed by Western blot using the Diagenode antibody against H3K18ac (Cat. No. CS-139-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk (lane 1). Lane 2 shows a coomassie blue staining of the gel. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Dot blotting Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet H3K18ac CS-139-100 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K18ac polyclonal antibody - Classic (Diagenode Cat# C15310139 Lot# A612-001 ). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Molecular analysis of enhancer RNAs and chromatin modifications in the region of their synthesis in Drosophila cells possessing genetic constructs.
    Fedoseeva DM, Kretova OV, Tchurikov NA

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