Diagenode

H2Bpan polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410157
(pAb-157-050)
50 μg/34 μl
$295.00
  Bulk order

Polyclonal antibody raised in rabbit against histone H2B using a KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminal part of the protein.

LotA616-004
Concentration1.46 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2 μg/IP Fig 1
ELISA 1:500 Fig 2
Dot Blotting 1:20,000 Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP

    Figure 1 ChIP results obtained with the Diagenode antibody directed against H2Bpan
    ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2Bpan (Cat. No. pAb-157-050) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (Cat. No. kch-maglow-016) on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive MYOD gene. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ELISA

    Figure 2 Determination of the titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H2Bpan (Cat. No. pAb-157-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:14,950.

    Dot Blot

    Figure 3 Cross reactivity test using the Diagenode antibody directed against H2Bpan
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H2Bpan (Cat. No. pAb-157-050) with the peptide used for immunization of the rabbit and other peptides containing unmodified sequences of different histones. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the H2B peptide.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DB
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H2Bpan pAb-157-050 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H2Bpan polyclonal antibody - Classic (Diagenode Cat# C15410157 Lot# A616-004). Click here to copy to clipboard.

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