Diagenode

ERR alpha polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410229-100
100 μl
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against ERR alpha (estrogen-related receptor alpha), using a recombinant protein.

Lot40471
Concentration0.93 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP assay 5 μg Fig 3
Immunoprecipitation 1:100 - 1:500 Fig 1
Western Blotting 1:500 - 1:3,000 Fig 2
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    IP

    Figure 1. ERR alpha antibody IP results
    ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of ERR alpha protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG (Cat. No. C15410206) C: Immunoprecipitation of ERR alpha by 2.5 μg of ERR alpha antibody (Cat. No. C15410229). The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody diluted 1:1,000.

    Western blot

    Figure 2. Western blot
    Sample: 30 μg of whole cell lysate (293T cells) 10% SDS PAGE C15410229 diluted 1:2,000

    ChIP

    Figure 3. ERR alpha antibody ChIP results
    ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter used as positive control or GAPDH promoter, used as negative control.

  • Applications
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet ERR C15410229 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ERR alpha polyclonal antibody - Classic (Diagenode Cat# C15410229-100 Lot# 40471). Click here to copy to clipboard.

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