Diagenode

DNMT3B polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410076
(pAb-076-050)
50 µg/50 µl
$295.00
  Bulk order
OUT OF STOCK

As an alternative we offer the purified DNMT3B polyclonal antibody - Classic (C15410218).

Please contact us for more information

Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.

LotA16-0042
Concentration1.0 µg/µl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 1 μg/ChIP Fig 1
ELISA 1:1,000 Fig 2
  • Validation Data

    ChIP

    Figure 1. ChIP using the Diagenode antibody against DNMT3B
    ChIP assays were performed using GDP control cells, the Diagenode antibody against DNMT3B (Cat. No. pAb-076-005) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 1 million cells using the “iDeal ChIP-seq” kit (Cat. No. AB-001-0024). A titration of the antibody consisting of 1, 2, and 5 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with two different primer pairs for the PRRX1 gene, used as a positive control target. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ELISA

    Figure 2. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against mouse DNMT3B (Cat. No. pAb-076-005), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:32,400.

  • Target description

    DNMT3B (UniProtKB/Swiss-Prot entry Q9UBC3) catalyses the genome wide de novo methylation of CpG residues, which regulates gene expression. DNMT3B is essential for development. DNA methylation on CpG residues is coordinated with methylation of histones. Six different isoforms of DNMT3B, produced by alternative splicing, exist although isoforms 4 and 5 may not be functional due to the absence of two conserved methyltransferase motifs.

     

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet DNMT3B pAb-076-005 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: DNMT3B polyclonal antibody - Classic (Diagenode Cat# C15410076 Lot# A16-0042). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    MCPH1 maintains long-term epigenetic silencing of ANGPT2 in chronic lymphocytic leukemia.
    Kopparapu PK, Miranda C, Fogelstrand L, Mishra K, Andersson PO, Kanduri C, Kanduri M
    The microcephalin gene (MCPH1) [also known as inhibitor of human telomerase reverse transcriptase (hTERT) expression] is a tumor suppressor gene that is functionally involved in the DNA damage response. Angiopoietin 2 (ANGPT2) is a crucial factor regulating tumor angiopoiesis. Deregulation of angiogenesis is one of ...

    A key role for EZH2 in epigenetic silencing of HOX genes in mantle cell lymphoma.
    Kanduri M, Sander B, Ntoufa S, Papakonstantinou N, Sutton LA, Stamatopoulos K, Kanduri C, Rosenquist R
    The chromatin modifier EZH2 is overexpressed and associated with inferior outcome in mantle cell lymphoma (MCL). Recently, we demonstrated preferential DNA methylation of HOX genes in MCL compared with chronic lymphocytic leukemia (CLL), despite these genes not being expressed in either entity. Since EZH2 has been s...

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