DNMT1 polyclonal antibody - Pioneer

Catalog Number
100 µl
  Bulk order

Alternative names: DNMT, MCMT, CXXC9, AIM 

Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.

Concentrationnot determined
Species reactivityMouse
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:1,000 Fig 1
Western Blotting 1:500 Fig 2
  • Validation Data


    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-077-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 44,800

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1
    Western blot was performed on nuclear extracts from N2A cells (N2A NE, 60 μg) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-077-100), diluted 1:500 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

  • Target description

    DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet DNMT1 CS-077-100 DATASHEET
    Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), ...
    Antibodies you can trust POSTER
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: DNMT1 polyclonal antibody - Pioneer (Diagenode Cat# C15310077 Lot# A13-002). Click here to copy to clipboard.

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