Diagenode

DNMT1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410227-100
100 μl
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against DNMT1 (DNA (cytosine-5-)-methyltransferase 1), using a KLH-conjugated synthetic peptide.

Lot40135
Concentration0.74 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 6
ICC/IF 1:100 - 1:1,000 Fig 2
IHC 1:100 - 1:1,000 Fig 1
Immunoprecipitation 1:100 - 1:500 Fig 5
Western Blotting 1:500 - 1:3,000 Fig 3, 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    IHC

    Figure 1. IHC
    Immunohistochemical analysis of paraffin-embedded PC3 Xenograft, using DNMT1 (C15410227) antibody at a 1:100 dilution.

    Immunofluorescence

    Figure 2. IF
    Immunofluorescence analysis of paraformaldehyde-fixed A431, using DNMT1 (C15410227) antibody at a 1:200 dilution.

    Western blot figure 1

    Figure 3. Western blot
    DNMT1 antibody detects DNMT1 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431 whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 5 % SDS-PAGE DNMT1 antibody (C15410227) dilution: 1:500

    Western blot figure 2

    Figure 4. Western blot
    DNMT1 antibody detects DNMT1 protein by western blot analysis. A. 30 μg Jurkat whole cell extract B. 30 μg Raji whole cell extract C. 30 μg NCI-H929 whole cell extract 5 % SDS-PAGE DNMT1 antibody (C15410227) dilution: 1:500

    IP

    Figure 5. IP
    DNMT1 antibody immunoprecipitates DNMT1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody (C15410227) 5 % SDS-PAGE The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody (C15410227) diluted 1:1,000.

    ChIP result

    Figure 6. ChIP
    ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2. The results are expressed as fold enrichment over the IgG negative control.

  • Target description

    DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet DNMT1 C15410227 DATASHEET
    Polyclonal antibody raised in rabbit against DNMT1 (DNA (cytosine-5-)-methyltransferase 1), using...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: DNMT1 polyclonal antibody - Classic (Diagenode Cat# C15410227-100 Lot# 40135). Click here to copy to clipboard.

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