Diagenode

CTNNB1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410222-100
100 μl
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against CTNNB1 (Catenin Beta 1), using a recombinant protein. 

Lot42235
Concentration0.1 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP* 5 μg/ChIP Fig 1
Western blot 1:1,000 - 1:3,000 Fig 2, 3
Immunoprecipitation 5 μg per IP Fig 4
Immunofluorescence 1:500 Fig 5
Immunohistochemistry 1:500 Fig 6

* Optimal dilutions/concentrations should be determined by the researcher.

  • Validation Data

    IHC

    Figure 1. IChIP results obtained with the Diagenode antibody directed against CTNNB1
    ChIP assays were performed using HCT116 cells and 5 μg of the Diagenode antibody against CTNNB1 (Cat. No. C15410222). An equal amount of IgG was used as a negative IP control. QPCR was performed with primers specific for the c-Myc promoter. Figure 1 shows the fold enrichment over the IgG negative control.

    IHC

    Figure 2. Western blot analysis using the Diagenode antibody directed against CTNNB1
    Whole cell extracts (30 μg) from A549 (lane1), H1299 (lane 2) and HCT116 (lane 3) cells were analysed by Western blot using the Diagenode antibody against CTNNB1 (Cat. No. C15410222) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IHC

    Figure 3. Western blot analysis using the Diagenode antibody directed against CTNNB1
    Whole cell extracts (30 μg) from HeLa cells transfected with CTNNB1 shRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against CTNNB1 (Cat. No. C15410222) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Alpha tubulin was used as a loading control (lower panel).

    IHC

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against CTNNB1
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 5 μg of the Diagenode antibody against CTNNB1 (Cat. No. C15410222, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated CTNNB1 protein was detected by western blot with the CTNNB1 antibody diluted 1:1,000.

    IHC

    Figure 5. IImmunofluorescence with the Diagenode antibody directed against CTNNB1
    HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against CTNNB1 (Cat. C15410222) diluted 1:500 (green). The cell nuclei were stained with Hoechst 33342 (blue).

    IHC

    Figure 6. Immunohistochemistry using the Diagenode antibody directed against CTNNB1
    Formalin fixed paraffin embedded human cervix (figure A), mouse intestine (figure B) and rat colon (figure C) were stained with the Diagenode antibody against CTNNB1 (Cat. No. C15410222) diluted 1:500, followed by a peroxidase labelled goat anti-rabbit secondary antibody.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet CTNNB1 C15410222 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CTNNB1 polyclonal antibody - Classic (Diagenode Cat# C15410222-100 Lot# 42235). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  • Related products

Events

 See all events

Twitter feed

News

 See all news