Diagenode

CTNNB1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410222-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against beta Catenin (catenin (cadherin-associated protein), beta 1, 88kDa), using a recombinant protein.

Lot41801
Concentration1 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 7
ICC/IF 1:100 - 1:1,000 Fig 9
IHC 1:100 - 1:1,000 Fig 1, 2, 3
Immunoprecipitation 1:100 - 1:500 Fig 8
Western Blotting 1:500 - 1:3,000 Fig 4, 5, 6
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    IHC

    Figure 1. IHC results obtained with the CTNNB1 antibody
    CTNNB1 antibody detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. The CTNNB1 antibody (Cat. No. C15410222) was used at a dilution of 1:500.

    IHC

    Figure 2. IHC results obtained with the CTNNB1 antibody
    CTNNB1 antibody detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. The CTNNB1 antibody (Cat. No. C15410222) was used at a dilution of 1:500.

    IHC

    Figure 3. IHC results obtained with the CTNNB1 antibody
    Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CTNNB1 (C15410222) antibody at a 1:500 dilution.

    Western blot

    Figure 4. Western blot
    Sample: 30 μg of HeLa whole cell lysate 7.5% SDS PAGE C15410222 diluted 1:1,000

    Western blot

    Figure 5. Western blot
    Sample: 50 μg of mouse brain whole cell lysate 7.5% SDS PAGE C15410222 diluted 1:1,000

    Western blot

    Figure 6. Western blot
    Sample: 30 μg PC-12 whole cell lysate 7.5 % SDS-PAGE CTNNB1 antibody (Cat. No. C15410222) dilution: 1:1,000

    ChIP

    Figure 7. CTNNB1 antibody ChIP results
    ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or CTNNB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.

    IP

    Figure 8. CTNNB1 antibody IP results
    CTNNB1 antibody immunoprecipitates CTNNB1 protein in IP experiments. IP samples: HeLa whole cell extract A. 30 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of CTNNB1 protein by 4 μg CTNNB1 antibody (Cat. No. C15410222) 5 % SDS-PAGE The immunoprecipitated CTNNB1 protein was detected by CTNNB1 antibody (Cat. No. C15410222) diluted 1:1,000.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet CTNNB1 C15410222 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CTNNB1 polyclonal antibody - Classic (Diagenode Cat# C15410222-100 Lot# 41801). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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