Diagenode

CIITA polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410062
(pAb-062-050)
50 µg/50 µl
$295.00
  Bulk order

Alternative names: C2TA, MHC2TA

Polyclonal antibody raised in rabbit against human CIITA protein (MHC class II transactivator), using a recombinant protein.

Lot001
Concentration1.0 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5-7 μg/IP Fig 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation Data

    ChIP results

    Figure 1 ChIP results obtained with the Diagenode antibody directed against CIITA
    ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the Diagenode antibody against CIITA (Cat. No. pAb-062-050) and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 μg of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure 1 shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).

  • Applications
  • Documents
    Datasheet CIITA pAb-062-050 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CIITA polyclonal antibody - Classic (Diagenode Cat# C15410062 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Genome-wide CIITA-binding profile identifies sequence preferences that dictate function versus recruitment.
    Scharer CD, Choi NM, Barwick BG, Majumder P, Lohsen S, Boss JM
    The class II transactivator (CIITA) is essential for the expression of major histocompatibility complex class II (MHC-II) genes; however, the role of CIITA in gene regulation outside of MHC-II biology is not fully understood. To comprehensively map CIITA-bound loci, ChIP-seq was performed in the human B lymphoblasto...

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