In eukaryotic cells DNA is associated with histones to form chromatin. The basic unit of chromatin is the nucleosome consisting of 146 bp of DNA wrapped around an octamer of the four core histones H2A, H2B, H3 and H4. Chromatin assembly is a fundamental process that is tightly linked to DNA replication and enables the cell to faithfully duplicate the chromosomes. Chromatin assembly can also occur independently of replication to turn-over histones, for instance during transcription or DNA damage repair. The chromatin-remodeling factor ACF (Acf1/ISWI complex) cooperates with the histone chaperone NAP-1 to assemble, in vitro, a regularly spaced chromatin in an ATP-dependent manner.
The kit contains recombinant human histones, the recombinant histone chaperone NAP‐1 and the recombinant Acf1/ ISWI complex, which catalyzes the deposition of histones into a regulary spaced nucleosome arrays in an ATP-dependent manner.