Diagenode

CBP polyclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410224-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against CBP (CREB binding protein), using a recombinant protein.

Lot39721
Concentration0.66 μg/μl
Species reactivityHuman, mouse, monkey
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP * 5 μg/ChIP Fig 1, 2
Western Blotting 1:500 - 1:3,000 Fig 3
Immunoprecipitation 2.5 μg/IP Fig 4
Immunofluorescence 1:100 - 1:1,000 Fig 5
Immunohistochemistry 1:100 - 1:1,000 Fig 6

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against CBP
    ChIP assays were performed using HeLa cells, the Diagenode antibody against CBP (Cat. No. C15410224) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/ IP) was used as negative IP control. QPCR was performed with primers for the ANKRD32 and IRS2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as a negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq

    ChIP-seq

    ChIP-seq

    ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBP
    ChIP was performed on sheared chromatin from 4 million HeLa cells using 5 μg of the Diagenode antibody against CBP (Cat. No. C15410224) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 2 Mb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ANKRD32 and IRS2 positive control genes (fig 2C and D).

    Western blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against CBP
    Whole cell extracts from H1299 cells (30 μg) were analysed by Western blot using the Diagenode antibody against CBP (Cat. No. C15410224) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunoprecipitation

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBP
    Immunoprecipitation was performed on whole cell extracts from HeLa cells (1 mg) using 2.5 μg of the Diagenode antibody against CBP (Cat. No. C15410224). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated CBP protein was detected by western blot with the CBP antibody diluted 1:1,000. The IP with the CBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract).

    Immunofluorescence

    Figure 5. Immunofluorescence with the Diagenode antibody directed against CBP
    HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against CBP (Cat. C15410224) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain.

    Immunohistochemistry

    Figure 6. Immunohistochemistry with the Diagenode antibody directed against CBP
    Formalin fixed paraffin embedded human cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue or mouse muscle (fig 6C), forebrain (fig 6D) and skin (fig 6E) tissue was stained with the Diagenode antibody against CBP (cat. No. C15410224) diluted 1:750 followed by a peroxidase labelled goat anti-rabbit secondary antibody.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet CBP C15410224 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

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