Diagenode

BRN2 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410223-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against Brn2 (POU class 3 homeobox 2), using a recombinant protein.

Lot40212
Concentration1 μg/μl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000
IHC 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 3
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. ChIP
    Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T chromatin extract A. 5 μg preimmune rabbit IgG B. 5 μg of Brn2 antibody (C15410223) The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.

    IP

    Figure 2. IP
    Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell extract A. 50 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 μg of Brn2 antibody (C15410223) The immunoprecipitated Brn2 protein was detected by 10% SDS-PAGE using the Brn2 antibody (C15410223) diluted 1:1,000.

    Western blot

    Figure 3. Western blot
    Brn2 antibody detects Brn2 protein by Western blot analysis. 30 μg A375 whole cell extract 10 % SDS-PAGE Brn2 antibody (C15410223) dilution: 1:1,000

    IHC

    Figure 4. IHC
    Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2 (C15410223) antibody at a 1:500 dilution.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet Brn2 C15410223 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: BRN2 polyclonal antibody - Classic (Diagenode Cat# C15410223-100 Lot# 40212). Click here to copy to clipboard.

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