BCL6 polyclonal antibody - Classic

Catalog Number
100 μg
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Polyclonal antibody raised in rabbit against BCL6 (B-cell CLL/lymphoma 6), using a recombinant protein.

Concentration1 μg/μl
Species reactivityHuman, mouse, rat
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP 5 μg/ChIP Fig 5
IHC 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:100 - 1:500 Fig 1
Western Blotting 1:500 - 1:3,000 Fig 2, 3
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data


    Figure 1. Immunoprecipitation
    BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell extract A: 30 μg whole cell extract of BCL6 protein expressing Raji cells B: Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody. The immunoprecipitated BCL6 protein was detected by Western blot with the BCL6 antibody diluted 1:1,000.

    Western blot

    Figure 2. Western blot results
    Sample: 30 μg of whole cell lysate A: Jurkat B: Raji C: NCI-H929 7.5% SDS PAGE BCL6 antibody diluted 1:1,000

    Western blot

    Figure 3. Western blot results
    BCL6 antibody detects BCL6 protein by Western blot analysis. A. 30 μg Neuro2A whole cell extract B. 30 μg GL261 whole cell extract C. 30 μg C8D30 whole cell extract D. 30 μg NIH-3T3 whole cell extract E. 30 μg Raw264.7 whole cell extract F. 30 μg C2C12 whole cell extract 7.5 % SDS-PAGE The BCL6 antibody (Cat. No. C15410221) was used at a dilution of 1:1,000


    Figure 4. IHC
    Immunohistochemical analysis of paraffin-embedded Tonsil, using BCL6 (C15410221) antibody (10 μg/ml).


    Figure 5. ChIP
    ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.Results are expressed as fold enrichment over the IgG negative control.

  • Applications
    Immunohistochemistry Read more
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet BCL6 C15410221 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: BCL6 polyclonal antibody - Classic (Diagenode Cat# C15410221-100 Lot# 40163). Click here to copy to clipboard.

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