Diagenode

Auto iDeal ChIP-seq Kit for Transcription Factors

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Catalog Number
Format
Price
C01010172
100 rxns
$1,995.00
Other format

Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. Unlike competing solutions, our kit utilizes a highly optimized protocol and is backed by validation with a broad number and range of transcription factors. The kit provides high yields with excellent specificity and sensitivity.

  • Characteristics
    • Confidence in results: Validated for ChIP-seq with multiple transcription factors
    • Proven: Validated by the epigenetics community, including the BLUEPRINT consortium
    • Most complete kit available for highest quality data - includes control antibodies and primers
    • Validated with Diagenode's High Resolution Library Preparation Kit and IP-Star® Automation

     

    Chromatin immunoprecipitation

    Diagenode peaks - overlapping peaks with encode dataset

    Figure 1. (A) Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade CTCF antibody. The IP'd DNA was subsequently analysed on an Illumina® HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in a region surrounding the GAPDH positive control gene. (B) The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.

     

    ChIP-seq figure A

    ChIP-seq figure B

    ChIP-seq figure C

    Figure 2. Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade HDAC1 (A), LSD1 (B) and p53 antibody (C). The IP'd DNA was subsequently analysed on an Illumina® Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in regions of chromosome 3 (A), chromosome 12 (B) and chromosome 6 (C) respectively.

  • Applications
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a ... Read more
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  • Documents
    Auto iDeal ChIP-seq kit for Transcription Factors MANUAL
    The Auto iDeal ChIP-seq kit for Transcription Factors was developed to enhance the utility ...
    Download
    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Auto iDeal ChIP-seq Kit for Transcription Factors (Diagenode Cat# C01010172). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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