Diagenode

CXXC1 polyclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410315
50 μg /50 μl
$295.00
  Bulk order

Polyclonal antibody raised in rabbit against human CXXC1 (CXXC Finger Protein 1), using a recombinant protein. 

Lot001
Concentration1 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2 μg/ChIP Fig 1, 2
Western Blotting 1:1,000 Fig 3
Immunofluorescence 1:100 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against CXXC1
    ChIP assays were performed using HeLa cells, the Diagenode antibody against CXXC1 (Cat. No. C15410315) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active EIF4A2 and GAPDH promoters, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CXXC1
    ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 μg of the Diagenode antibody against CXXC1 (Cat. No. C15410315) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and an 800 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the EIF4A2 and GAPDH positive control genes, respectively (figure 2C and D).

    Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against CXXC1
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CXXC1 (Cat. No. C15410315). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunofluorescence

    Figure 4. Immunofluorescence using the Diagenode antibody directed against CXXC1
    A549 cells were stained with the Diagenode antibody against CXXC1 (Cat. No. C15410315) diluted 1:100. The right picture shows costaining with DAPI.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-seq (ab)
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    ChIP-qPCR (ab)
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  • Documents
    Datasheet CXXC1 polyclonal antibody - C15410315 DATASHEET
    Datasheet of CXXC1 polyclonal antibody
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CXXC1 polyclonal antibody - Classic (Diagenode Cat# C15410315 Lot# 001). Click here to copy to clipboard.

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  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
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    Boston, USA
    Oct 3-Oct 4, 2016
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