Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'en',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'product' => array(
'Product' => array(
'id' => '2627',
'antibody_id' => null,
'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
'created' => '2015-06-29 14:08:20',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
)
$language = 'en'
$meta_keywords = ''
$meta_description = '0.5 ml Bioruptor® Microtubes'
$meta_title = '0.5 ml Bioruptor® Microtubes'
$product = array(
'Product' => array(
'id' => '2627',
'antibody_id' => null,
'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
'created' => '2015-06-29 14:08:20',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
'id' => '1809',
'antibody_id' => null,
'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '13',
'position' => '10',
'parent_id' => '3',
'name' => 'DNA/RNA shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
</div>
<p></p>
<div class="row">
<div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div>
<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
</div>
<p><br /><br /></p>
<div class="row">
<div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div>
<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
</div>
<p><br /><br /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div>
<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'dna-rna-shearing',
'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ',
'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.',
'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode',
'modified' => '2017-12-08 14:44:11',
'created' => '2014-10-29 12:45:41',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'chromatin-shearing',
'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator',
'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
'modified' => '2017-11-15 10:14:02',
'created' => '2015-03-05 15:56:30',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '67',
'position' => '1',
'parent_id' => '6',
'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories',
'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'bioruptor-plus-accessories',
'cookies_tag_id' => null,
'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator',
'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus',
'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode',
'modified' => '2015-10-21 09:57:14',
'created' => '2015-07-23 11:39:03',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '776',
'name' => 'Datasheet 05microtubes WA-004-0500',
'description' => 'Datasheet description',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor/Datasheet_05microtubes_WA-004-0500.pdf',
'slug' => 'datasheet-05microtubes-wa-004-0500',
'meta_keywords' => null,
'meta_description' => null,
'modified' => '2015-07-07 11:47:45',
'created' => '2015-07-07 11:47:45',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '140',
'name' => 'product/shearing_technologies/C30010013_tube.png',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '18',
'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus',
'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>',
'image_id' => '214',
'type' => 'Protocol',
'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf',
'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:41:17',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200043_tube_holder.jpg" alt="Product image" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200043</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1809" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1809" id="CartAdd/1809Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1809" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1809" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5/0.65 ml tube holder for Bioruptor® Stan...</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '1809',
'antibody_id' => null,
'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
'id' => '4628',
'product_id' => '2627',
'related_id' => '1809'
),
'Image' => array(
(int) 0 => array(
'id' => '249',
'name' => 'product/shearing_technologies/B01200043_tube_holder.jpg',
'alt' => 'Product image',
'modified' => '2015-07-09 15:54:41',
'created' => '2015-07-09 15:54:41',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(WA-004-0500)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '108',
'product_id' => '2627',
'protocol_id' => '33'
)
)
$document = array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
'id' => '729',
'product_id' => '2627',
'document_id' => '791'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'en',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'product' => array(
'Product' => array(
'id' => '2627',
'antibody_id' => null,
'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
'created' => '2015-06-29 14:08:20',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
)
$language = 'en'
$meta_keywords = ''
$meta_description = '0.5 ml Bioruptor® Microtubes'
$meta_title = '0.5 ml Bioruptor® Microtubes'
$product = array(
'Product' => array(
'id' => '2627',
'antibody_id' => null,
'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
'created' => '2015-06-29 14:08:20',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
'id' => '1809',
'antibody_id' => null,
'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '13',
'position' => '10',
'parent_id' => '3',
'name' => 'DNA/RNA shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
</div>
<p></p>
<div class="row">
<div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div>
<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
</div>
<p><br /><br /></p>
<div class="row">
<div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div>
<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
</div>
<p><br /><br /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div>
<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'dna-rna-shearing',
'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ',
'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.',
'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode',
'modified' => '2017-12-08 14:44:11',
'created' => '2014-10-29 12:45:41',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'chromatin-shearing',
'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator',
'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
'modified' => '2017-11-15 10:14:02',
'created' => '2015-03-05 15:56:30',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '67',
'position' => '1',
'parent_id' => '6',
'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories',
'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'bioruptor-plus-accessories',
'cookies_tag_id' => null,
'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator',
'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus',
'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode',
'modified' => '2015-10-21 09:57:14',
'created' => '2015-07-23 11:39:03',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '776',
'name' => 'Datasheet 05microtubes WA-004-0500',
'description' => 'Datasheet description',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor/Datasheet_05microtubes_WA-004-0500.pdf',
'slug' => 'datasheet-05microtubes-wa-004-0500',
'meta_keywords' => null,
'meta_description' => null,
'modified' => '2015-07-07 11:47:45',
'created' => '2015-07-07 11:47:45',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '140',
'name' => 'product/shearing_technologies/C30010013_tube.png',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '18',
'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus',
'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>',
'image_id' => '214',
'type' => 'Protocol',
'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf',
'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:41:17',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200043_tube_holder.jpg" alt="Product image" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200043</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1809" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1809" id="CartAdd/1809Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1809" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1809" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5/0.65 ml tube holder for Bioruptor® Stan...</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '1809',
'antibody_id' => null,
'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
'id' => '4628',
'product_id' => '2627',
'related_id' => '1809'
),
'Image' => array(
(int) 0 => array(
'id' => '249',
'name' => 'product/shearing_technologies/B01200043_tube_holder.jpg',
'alt' => 'Product image',
'modified' => '2015-07-09 15:54:41',
'created' => '2015-07-09 15:54:41',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(WA-004-0500)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '108',
'product_id' => '2627',
'protocol_id' => '33'
)
)
$document = array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
'id' => '729',
'product_id' => '2627',
'document_id' => '791'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'en',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'product' => array(
'Product' => array(
'id' => '2627',
'antibody_id' => null,
'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
'created' => '2015-06-29 14:08:20',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
)
$language = 'en'
$meta_keywords = ''
$meta_description = '0.5 ml Bioruptor® Microtubes'
$meta_title = '0.5 ml Bioruptor® Microtubes'
$product = array(
'Product' => array(
'id' => '2627',
'antibody_id' => null,
'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
'created' => '2015-06-29 14:08:20',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
'id' => '1809',
'antibody_id' => null,
'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '13',
'position' => '10',
'parent_id' => '3',
'name' => 'DNA/RNA shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
</div>
<p></p>
<div class="row">
<div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div>
<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
</div>
<p><br /><br /></p>
<div class="row">
<div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div>
<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
</div>
<p><br /><br /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div>
<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'dna-rna-shearing',
'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ',
'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.',
'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode',
'modified' => '2017-12-08 14:44:11',
'created' => '2014-10-29 12:45:41',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'chromatin-shearing',
'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator',
'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
'modified' => '2017-11-15 10:14:02',
'created' => '2015-03-05 15:56:30',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '67',
'position' => '1',
'parent_id' => '6',
'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories',
'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'bioruptor-plus-accessories',
'cookies_tag_id' => null,
'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator',
'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus',
'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode',
'modified' => '2015-10-21 09:57:14',
'created' => '2015-07-23 11:39:03',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '776',
'name' => 'Datasheet 05microtubes WA-004-0500',
'description' => 'Datasheet description',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor/Datasheet_05microtubes_WA-004-0500.pdf',
'slug' => 'datasheet-05microtubes-wa-004-0500',
'meta_keywords' => null,
'meta_description' => null,
'modified' => '2015-07-07 11:47:45',
'created' => '2015-07-07 11:47:45',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '140',
'name' => 'product/shearing_technologies/C30010013_tube.png',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '18',
'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus',
'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>',
'image_id' => '214',
'type' => 'Protocol',
'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf',
'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:41:17',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200043_tube_holder.jpg" alt="Product image" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200043</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1809" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1809" id="CartAdd/1809Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1809" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1809" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5/0.65 ml tube holder for Bioruptor® Stan...</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '1809',
'antibody_id' => null,
'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
'id' => '4628',
'product_id' => '2627',
'related_id' => '1809'
),
'Image' => array(
(int) 0 => array(
'id' => '249',
'name' => 'product/shearing_technologies/B01200043_tube_holder.jpg',
'alt' => 'Product image',
'modified' => '2015-07-09 15:54:41',
'created' => '2015-07-09 15:54:41',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(WA-004-0500)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '108',
'product_id' => '2627',
'protocol_id' => '33'
)
)
$document = array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
'id' => '729',
'product_id' => '2627',
'document_id' => '791'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'en',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'product' => array(
'Product' => array(
'id' => '2627',
'antibody_id' => null,
'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
'created' => '2015-06-29 14:08:20',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
)
$language = 'en'
$meta_keywords = ''
$meta_description = '0.5 ml Bioruptor® Microtubes'
$meta_title = '0.5 ml Bioruptor® Microtubes'
$product = array(
'Product' => array(
'id' => '2627',
'antibody_id' => null,
'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
'created' => '2015-06-29 14:08:20',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
'id' => '1809',
'antibody_id' => null,
'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '13',
'position' => '10',
'parent_id' => '3',
'name' => 'DNA/RNA shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
</div>
<p></p>
<div class="row">
<div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div>
<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
</div>
<p><br /><br /></p>
<div class="row">
<div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div>
<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
</div>
<p><br /><br /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div>
<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'dna-rna-shearing',
'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ',
'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.',
'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode',
'modified' => '2017-12-08 14:44:11',
'created' => '2014-10-29 12:45:41',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'chromatin-shearing',
'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator',
'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
'modified' => '2017-11-15 10:14:02',
'created' => '2015-03-05 15:56:30',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '67',
'position' => '1',
'parent_id' => '6',
'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories',
'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'bioruptor-plus-accessories',
'cookies_tag_id' => null,
'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator',
'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus',
'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode',
'modified' => '2015-10-21 09:57:14',
'created' => '2015-07-23 11:39:03',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '776',
'name' => 'Datasheet 05microtubes WA-004-0500',
'description' => 'Datasheet description',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor/Datasheet_05microtubes_WA-004-0500.pdf',
'slug' => 'datasheet-05microtubes-wa-004-0500',
'meta_keywords' => null,
'meta_description' => null,
'modified' => '2015-07-07 11:47:45',
'created' => '2015-07-07 11:47:45',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '140',
'name' => 'product/shearing_technologies/C30010013_tube.png',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '18',
'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus',
'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>',
'image_id' => '214',
'type' => 'Protocol',
'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf',
'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:41:17',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200043_tube_holder.jpg" alt="Product image" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200043</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1809" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1809" id="CartAdd/1809Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1809" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1809" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5/0.65 ml tube holder for Bioruptor® Stan...</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '1809',
'antibody_id' => null,
'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
'id' => '4628',
'product_id' => '2627',
'related_id' => '1809'
),
'Image' => array(
(int) 0 => array(
'id' => '249',
'name' => 'product/shearing_technologies/B01200043_tube_holder.jpg',
'alt' => 'Product image',
'modified' => '2015-07-09 15:54:41',
'created' => '2015-07-09 15:54:41',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(WA-004-0500)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '108',
'product_id' => '2627',
'protocol_id' => '33'
)
)
$document = array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
'id' => '729',
'product_id' => '2627',
'document_id' => '791'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
