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Diagenode Launches a Unique Portfolio for RNA Sequencing Library Preparation
October 27, 2016

Diagenode, a leading global provider for complete solutions for sample preparation and epigenetics research, recently launched a complete product solution for RNA sequencing using “Capture and Amplification by Tailing and Switching (CATS), complementing the company’s current NGS library preparation portfolio. The company is initially launching the library preparation kit for sequencing small RNAs – the CATS Small RNA-seq Kit. Library preparation kits available through the company’s early access program include those for the transcriptome (mRNAs only) and whole transcriptome (long non-coding RNAs and mRNAs). Full access will begin by the end of the year.

CATS is a ligation-independent method for generating DNA libraries for next generation sequencing from low input amounts of DNA or RNA. CATS does not require adaptor ligation, increasing the overall efficiency and yields of the protocol. The Diagenode kits generate ready-to-sequence RNA-seq libraries from picogram amounts of RNA in just a few hours.

“Our CATS protocol will become the method of choice for RNA sequencing,” stated Raphael Werding, Senior Product Manager at Diagenode. “The CATS Small RNA-seq Kit, which is now broadly available, permits RNA-sequencing from minimal inputs down to 10 picograms. Unlike competing solutions, Diagenode’s solution exhibits better library efficiency compared to ligation-based methods. The CATS method allows researchers to accurately identify more small RNAs from better template capture and lower bias due to lower amplification requirements.”

Werding also added that all of the CATS kits utilize a faster and easier protocol with reduced hands-on time than competing kits. “CATS eliminates the need for the numerous purifications and size selection from ligation-based methods.”

“Diagenode’s new RNA-sequencing product portfolio addresses the needs of customers looking for a cutting-edge library preparation technique for RNA,” said Didier Allaer, CEO of Diagenode. “Researchers will now have their choice of preparing libraries from a broad spectrum of RNAs.” Allaer also stated, “Non-coding RNAs play a very important role in epigenetic mechanisms, such as transcriptional or translational regulation, and our new solutions can help researchers understand such mechanisms.”

More information on Diagenode’s CATS RNA-sequencing products can be found here

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