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TFIIF polyclonal antibody - Classic

100 μl
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Polyclonal antibody raised in rabbit against TFIIF (general transcription factor IIF, polypeptide 1, 74kDa), using a recombinant protein.

Concentration1 μg/μl
Species reactivityHuman, mouse
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 3, 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP results

    Figure 1. TFIIF ChIP results
    ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.

    IP results

    Figure 2. TFIIF IP results
    TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of TFIIF protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C: Immunoprecipitation of TFIIF with 2.5 μg of TFIIF antibody (C15410256) The immunoprecipitated TFIIF protein was detected by western blot with the TFIIF antibody diluted 1:1,000.

    Western blot

    Figure 3. Western blot
    Sample: 30 μg of whole cell lysate A: 293T B: A431 C: HeLa 7.5% SDS PAGE TFIIF antibody dilution 1:3,000.

    Western blot

    Figure 4. Western blot
    Sample: 30 μg of NIH-3T3 whole cell lysate 7.5% SDS PAGE TFIIF antibody dilution 1:3,000

  •  実験手法
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet TFIIF C15410256 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TFIIF polyclonal antibody - Classic (Diagenode Cat# C15410256-100 Lot# 40723). Click here to copy to clipboard.

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