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Figure 1. TFIIF ChIP results ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
Figure 2. TFIIF IP results TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of TFIIF protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C: Immunoprecipitation of TFIIF with 2.5 μg of TFIIF antibody (C15410256) The immunoprecipitated TFIIF protein was detected by western blot with the TFIIF antibody diluted 1:1,000.
Figure 3. Western blot Sample: 30 μg of whole cell lysate A: 293T B: A431 C: HeLa 7.5% SDS PAGE TFIIF antibody dilution 1:3,000.
Figure 4. Western blot Sample: 30 μg of NIH-3T3 whole cell lysate 7.5% SDS PAGE TFIIF antibody dilution 1:3,000
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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