Diagenode

SMAD2 polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15410251-100
100 μl
$345.00
  Bulk order

Polyclonal antibody raised in rabbit against SMAD2 (SMAD family member 2), using a recombinant protein.

Lot40044
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
Immunoprecipitation 1:500 - 1:1,000 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. ChIP
    SMAD2 antibody immunoprecipitates SMAD2 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 antibody (C15410251). The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.

    Immunoprecipitation

    Figure 2. Immunoprecipitation
    SMAD2 antibody immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell extract A. 50 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2 protein by 2 μg of SMAD2 antibody (Cat. No. C15410251). The immunoprecipitated SMAD2 protein was detected by western blot with the SMAD2 antibody diluted 1:1,000.

    Immunofluorescence

    Figure 3. Immunofluorescence
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using SMAD2 (Cat. No. C15410251) antibody at a 1:200 dilution.

    Western Blot

    Figure 4. Western Blot
    Sample: 30 μg of Hela whole cell lysate 10% SDS PAGE C15410251 diluted 1:1000

  •  実験手法
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet SMAD2 C15410251 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMAD2 polyclonal antibody - Classic (Diagenode Cat# C15410251-100 Lot# 40044). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  関連商品

イベント

  • Genome instability: when RNA meets chromatin
    Roscoff
    Sep 16-Jun 20, 2019
  • Japanese Biochemical Society
    Yokohama
    Sep 18-Sep 20, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    Atlanta, Georgia
    Sep 19, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    RTP, North Carolina
    Sep 20, 2019
 すべてのイベントを見る

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics