Diagenode

PTBP1 polyclonal antibody

カタログ番号
フォーマット
価格
C15410354
100 µg
$315.00
  Bulk order



Other names: PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I

Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.

Lot001
Concentration1 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C.
Storage BufferPBS containing 50% glycerol, does not contain a preservative.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP 15 µg per 107 cells Fig 1
Western blotting 1:1,000 Fig 2
IP 5 µg per 2.5x106 cells Fig 3
  • Validation data

    ChIP

    RIP

    Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1
    Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    WB

    Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1
    Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (Cat. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IP

    Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1
    Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.

  •  実験手法
    RIP
    RIP Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  •  資料
    PTBP1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1)...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PTBP1 polyclonal antibody (Diagenode Cat# C15410354 Lot# 001). Click here to copy to clipboard.

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イベント

  • Molecular Biology Society of Japan
    Fukuoka
    Dec 3-Dec 6, 2019
  • ASH annual meeting
    Orlando, FL
    Dec 7-Dec 10, 2019
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