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Other names: PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I
Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C. |
| Storage Buffer | PBS containing 50% glycerol, does not contain a preservative. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| RIP | 15 µg per 107 cells | Fig 1 |
| Western blotting | 1:1,000 | Fig 2 |
| IP | 5 µg per 2.5x106 cells | Fig 3 |
Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1
Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1
Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (Cat. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1
Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.
| RIP RIP Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| PTBP1 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1)... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: PTBP1 polyclonal antibody (Diagenode Cat# C15410354 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'PTBP1 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2981', 'antibody_id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I</p><p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1</strong><br />Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410354', 'old_catalog_number' => '', 'sf_code' => 'C15410354-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'ptbp1-polyclonal-antibody-100-ul', 'meta_title' => 'PTBP1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:57:18', 'created' => '2018-06-28 15:13:09', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => 'PTBP1 (UniProtKB/Swiss-Prot entry P26599) is a heterogeneous nuclear ribonucleoprotein (hnRNP), a family of proteins which bind to heterogeneous nuclear RNA (hnRNA). PTBP1 is associated with pre-mRNAs in the nucleus and plays a role in pre-mRNA splicing and in the regulation of alternative splicing events and in other aspects of mRNA metabolism and transport. The PTBP1 protein has four repeats of quasi-RNA recognition motif (RRM) domains that bind to intronic polypyrimidine tracts.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. 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Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1</strong><br />Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410354', 'old_catalog_number' => '', 'sf_code' => 'C15410354-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'ptbp1-polyclonal-antibody-100-ul', 'meta_title' => 'PTBP1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:57:18', 'created' => '2018-06-28 15:13:09', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => 'PTBP1 (UniProtKB/Swiss-Prot entry P26599) is a heterogeneous nuclear ribonucleoprotein (hnRNP), a family of proteins which bind to heterogeneous nuclear RNA (hnRNA). PTBP1 is associated with pre-mRNAs in the nucleus and plays a role in pre-mRNA splicing and in the regulation of alternative splicing events and in other aspects of mRNA metabolism and transport. The PTBP1 protein has four repeats of quasi-RNA recognition motif (RRM) domains that bind to intronic polypyrimidine tracts.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4933', 'product_id' => '2981', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'jpn' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '1023', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/ptbp1-polyclonal-antibody-100-ul.pdf', 'slug' => 'ptbp1-polyclonal-antibody-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-16 12:25:47', 'created' => '2018-07-16 12:06:15', 'ProductsDocument' => array( 'id' => '2663', 'product_id' => '2981', 'document_id' => '1023' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'PTBP1 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2981', 'antibody_id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I</p><p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1</strong><br />Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410354', 'old_catalog_number' => '', 'sf_code' => 'C15410354-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'ptbp1-polyclonal-antibody-100-ul', 'meta_title' => 'PTBP1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:57:18', 'created' => '2018-06-28 15:13:09', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => 'PTBP1 (UniProtKB/Swiss-Prot entry P26599) is a heterogeneous nuclear ribonucleoprotein (hnRNP), a family of proteins which bind to heterogeneous nuclear RNA (hnRNA). PTBP1 is associated with pre-mRNAs in the nucleus and plays a role in pre-mRNA splicing and in the regulation of alternative splicing events and in other aspects of mRNA metabolism and transport. The PTBP1 protein has four repeats of quasi-RNA recognition motif (RRM) domains that bind to intronic polypyrimidine tracts.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'PTBP1 polyclonal antibody' $product = array( 'Product' => array( 'id' => '2981', 'antibody_id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I</p><p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1</strong><br />Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410354', 'old_catalog_number' => '', 'sf_code' => 'C15410354-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'ptbp1-polyclonal-antibody-100-ul', 'meta_title' => 'PTBP1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:57:18', 'created' => '2018-06-28 15:13:09', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => 'PTBP1 (UniProtKB/Swiss-Prot entry P26599) is a heterogeneous nuclear ribonucleoprotein (hnRNP), a family of proteins which bind to heterogeneous nuclear RNA (hnRNA). PTBP1 is associated with pre-mRNAs in the nucleus and plays a role in pre-mRNA splicing and in the regulation of alternative splicing events and in other aspects of mRNA metabolism and transport. The PTBP1 protein has four repeats of quasi-RNA recognition motif (RRM) domains that bind to intronic polypyrimidine tracts.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ptbp1-polyclonal-antibody ', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:32:08', 'created' => '2018-06-27 15:32:08', 'select_label' => '537 - PTBP1 polyclonal antibody (001 - 1 μg/μl - Human: positive. 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Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div> <p></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'mirna-sirna', 'cookies_tag_id' => null, 'meta_keywords' => 'miRNA & siRNA meta Polyclonal antibody,Monoclonal antibody', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing', 'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode', 'modified' => '2020-09-01 11:42:06', 'created' => '2014-11-12 02:34:56', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1023', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/ptbp1-polyclonal-antibody-100-ul.pdf', 'slug' => 'ptbp1-polyclonal-antibody-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-16 12:25:47', 'created' => '2018-07-16 12:06:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4933', 'product_id' => '2981', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'jpn' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '1023', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/ptbp1-polyclonal-antibody-100-ul.pdf', 'slug' => 'ptbp1-polyclonal-antibody-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-16 12:25:47', 'created' => '2018-07-16 12:06:15', 'ProductsDocument' => array( 'id' => '2663', 'product_id' => '2981', 'document_id' => '1023' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'PTBP1 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2981', 'antibody_id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I</p><p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1</strong><br />Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410354', 'old_catalog_number' => '', 'sf_code' => 'C15410354-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'ptbp1-polyclonal-antibody-100-ul', 'meta_title' => 'PTBP1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:57:18', 'created' => '2018-06-28 15:13:09', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => 'PTBP1 (UniProtKB/Swiss-Prot entry P26599) is a heterogeneous nuclear ribonucleoprotein (hnRNP), a family of proteins which bind to heterogeneous nuclear RNA (hnRNA). PTBP1 is associated with pre-mRNAs in the nucleus and plays a role in pre-mRNA splicing and in the regulation of alternative splicing events and in other aspects of mRNA metabolism and transport. The PTBP1 protein has four repeats of quasi-RNA recognition motif (RRM) domains that bind to intronic polypyrimidine tracts.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ptbp1-polyclonal-antibody ', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:32:08', 'created' => '2018-06-27 15:32:08', 'select_label' => '537 - PTBP1 polyclonal antibody (001 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'PTBP1 polyclonal antibody' $product = array( 'Product' => array( 'id' => '2981', 'antibody_id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I</p><p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1</strong><br />Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410354', 'old_catalog_number' => '', 'sf_code' => 'C15410354-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'ptbp1-polyclonal-antibody-100-ul', 'meta_title' => 'PTBP1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:57:18', 'created' => '2018-06-28 15:13:09', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => 'PTBP1 (UniProtKB/Swiss-Prot entry P26599) is a heterogeneous nuclear ribonucleoprotein (hnRNP), a family of proteins which bind to heterogeneous nuclear RNA (hnRNA). PTBP1 is associated with pre-mRNAs in the nucleus and plays a role in pre-mRNA splicing and in the regulation of alternative splicing events and in other aspects of mRNA metabolism and transport. The PTBP1 protein has four repeats of quasi-RNA recognition motif (RRM) domains that bind to intronic polypyrimidine tracts.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ptbp1-polyclonal-antibody ', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:32:08', 'created' => '2018-06-27 15:32:08', 'select_label' => '537 - PTBP1 polyclonal antibody (001 - 1 μg/μl - Human: positive. 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Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4933', 'product_id' => '2981', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'jpn' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '1023', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/ptbp1-polyclonal-antibody-100-ul.pdf', 'slug' => 'ptbp1-polyclonal-antibody-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-16 12:25:47', 'created' => '2018-07-16 12:06:15', 'ProductsDocument' => array( 'id' => '2663', 'product_id' => '2981', 'document_id' => '1023' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'PTBP1 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2981', 'antibody_id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I</p><p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1</strong><br />Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410354', 'old_catalog_number' => '', 'sf_code' => 'C15410354-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'ptbp1-polyclonal-antibody-100-ul', 'meta_title' => 'PTBP1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:57:18', 'created' => '2018-06-28 15:13:09', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => 'PTBP1 (UniProtKB/Swiss-Prot entry P26599) is a heterogeneous nuclear ribonucleoprotein (hnRNP), a family of proteins which bind to heterogeneous nuclear RNA (hnRNA). PTBP1 is associated with pre-mRNAs in the nucleus and plays a role in pre-mRNA splicing and in the regulation of alternative splicing events and in other aspects of mRNA metabolism and transport. The PTBP1 protein has four repeats of quasi-RNA recognition motif (RRM) domains that bind to intronic polypyrimidine tracts.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. 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Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'PTBP1 polyclonal antibody' $product = array( 'Product' => array( 'id' => '2981', 'antibody_id' => '537', 'name' => 'PTBP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> PTB, PTB-1, PTP-T, OTB2, PTB3, PTB4, PPTB, HNRPI, HNRNPI, HNRNP-I</p><p>Polyclonal antibody raised in rabbit against human PTBP1 (Polypyrimidine Tract Binding Protein 1), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1</strong><br />Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410354-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (<span>Cat</span>. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). 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PTBP1 is associated with pre-mRNAs in the nucleus and plays a role in pre-mRNA splicing and in the regulation of alternative splicing events and in other aspects of mRNA metabolism and transport. The PTBP1 protein has four repeats of quasi-RNA recognition motif (RRM) domains that bind to intronic polypyrimidine tracts.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. 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APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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