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Figure 1. Immunoprecipitation using the Diagenode antibody directed against PTBP1 Immunoprecipitation was performed on total RNA isolated from 6 million Jurkat cells using 15 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PTBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PTBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. Western blot analysis using the Diagenode antibody directed against PTBP1 Whole cell extracts from K562, 293T, Jurkat and HeLa cells (lanes 1 to 4, respectively) were analysed by Western blot using the Diagenode antibody against PTBP1 (Cat. No. C15410354) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunoprecipitation using the Diagenode antibody directed against PTBP1 Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 5 µg of the Diagenode antibody against PTBP1 (Cat. No. C15410354, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated PTBP1 protein was subsequently detected by western blot with the PTBP1 antibody as described above.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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