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PCBP1 polyclonal antibody

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Catalog Number

Format

Price

C15410359

100 µg

$360.00
Bulk order

Other names: HEL-S-85, HNRPE1, HNRPX, HnRP-E1, HnRP-X

Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.

Lot	004
Concentration	1 μg/μl
Species reactivity	Human, mouse, rat: positive. Other species: not tested.
Type	Polyclonal
Purity	Affinity purified polyclonal antibody.
Host	Rabbit
Storage Conditions	Store at -20°C.
Storage Buffer	PBS containing 50% glycerol, does not contain a preservative.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
RIP	15 µg per 10⁷ cells	Fig 1
Western blotting	1:1,000	Fig 2
IP	5 µg per 5x10⁶ cells	Fig 3

Validation data

Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1
Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1
Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (Cat. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1
Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode antibody against PCBP1 (Cat. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.

資料
PCBP1 polyclonal antibody datasheet DATASHEET
Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KL... Download

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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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			'antibody_id' => '542',
			'name' => 'PCBP1 polyclonal antibody',
			'description' => '<p><strong>Other names:</strong> HEL-S-85, HNRPE1, HNRPX, HnRP-E1, HnRP-X</p><p>Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.</p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-7 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tr>
<td>RIP</td>
<td>15 &micro;g per 10<sup>7</sup> cells</td>
<td>Fig 1</td>
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<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<tr>
<td>IP</td>
<td>5 &micro;g per 5x10<sup>6</sup> cells</td>
<td>Fig 3</td>
</tr>
</tbody>
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		'antibody_id' => '542',
		'name' => 'PCBP1 polyclonal antibody',
		'description' => '<p><strong>Other names:</strong> HEL-S-85, HNRPE1, HNRPX, HnRP-E1, HnRP-X</p><p>Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.</p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<div class="small-5 columns">
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<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-5 columns">
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<div class="small-7 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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		'meta_title' => 'PCBP1 polyclonal antibody',
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		'modified' => '2022-01-05 15:55:07',
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		'id' => '542',
		'name' => 'PCBP1 polyclonal antibody',
		'description' => 'PCBP1 (UniProtKB/Swiss-Prot entry Q15365) is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC. It is thought to play a role in formation of a sequence-specific alpha-globin mRNP complex which iinfluences alpha-globin mRNA stability. PCBP1 also promotes poliovirus RNA replication and has been implicated in translational control of the human Papillomavirus type 16 L2 mRNA and hepatitis A virus RNA.',
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		'concentration' => '1 μg/μl',
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		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>RIP</td>
<td>15 &micro;g per 10<sup>7</sup> cells</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>IP</td>
<td>5 &micro;g per 5x10<sup>6</sup> cells</td>
<td>Fig 3</td>
</tr>
</tbody>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
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			'id' => '2986',
			'antibody_id' => '542',
			'name' => 'PCBP1 polyclonal antibody',
			'description' => '<p><strong>Other names:</strong> HEL-S-85, HNRPE1, HNRPX, HnRP-E1, HnRP-X</p><p>Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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			'name' => 'PCBP1 antibody SDS BE fr',
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			'created' => '2020-06-08 16:49:47',
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	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
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	'created' => '2015-07-08 13:46:50',
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		'product_id' => '2986',
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	'parent_id' => '40',
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	'description' => '<p>Immunoprecipitation</p>',
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	'online' => true,
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	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
	'locale' => 'jpn'
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$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
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	'description' => '<p>Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
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$dataForView = array(
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	'meta_keywords' => '',
	'meta_description' => '',
	'meta_title' => 'PCBP1 polyclonal antibody',
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		'Product' => array(
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			'antibody_id' => '542',
			'name' => 'PCBP1 polyclonal antibody',
			'description' => '<p><strong>Other names:</strong> HEL-S-85, HNRPE1, HNRPX, HnRP-E1, HnRP-X</p><p>Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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<thead>
<tr>
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<tr>
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<td>Fig 1</td>
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<tr>
<td>Western blotting</td>
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<td>Fig 2</td>
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<tr>
<td>IP</td>
<td>5 &micro;g per 5x10<sup>6</sup> cells</td>
<td>Fig 3</td>
</tr>
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		'antibody_id' => '542',
		'name' => 'PCBP1 polyclonal antibody',
		'description' => '<p><strong>Other names:</strong> HEL-S-85, HNRPE1, HNRPX, HnRP-E1, HnRP-X</p><p>Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.</p>',
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		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
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<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410359-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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<th>Suggested dilution</th>
<th>References</th>
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<td>RIP</td>
<td>15 &micro;g per 10<sup>7</sup> cells</td>
<td>Fig 1</td>
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<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<td>IP</td>
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<td>Fig 3</td>
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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