p53K372me1 polyclonal antibody - Classic

50 µg/25 µl
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Alternative names: TP53, P53, TRP53, LSF1

Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), methylated at K372, using a KLH-conjugated synthetic peptide.

Concentration2.0 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:200 Fig 1
ChIP 5 μg/ChIP Ref 1
  • Validation Data

    Western blot

    Figure 1 Western blot analysis using the Diagenode antibody directed against p53K372me1
    Western blot was performed on 100 μg Colon carcinoma whole cell extract (HCT116 WCE) using the Diagenode antibody against p53K372me1 (Cat. No. pAb-048-050), diluted 1:200 in TBS-Tween containing 5% skimmed milk. The size and location of the protein of interest are indicated (Figure 1, kindly provided by Liu Xiangyu, Health Science Center, Peking University, China).

  •  実験手法
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    定量のPCRとクロマチン免疫沈降(ChIP)が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、およ... Read more
  •  資料
    Datasheet p53K372me1 pAb-048-050 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  出版物

    How to properly cite this product in your work

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