Diagenode

MeCP2 polyclonal antibody - Pioneer

カタログ番号
フォーマット
価格
C15310106
(CS-106-100)
100 µl
$210.00
  Bulk order



Alternative names: AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

Polyclonal antibody raised in rabbit against mouse MeCP2 (Methyl-CpG-binding domain Protein 2), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the N-terminal part of the protein.

LotA375-001
Concentrationnot determined
Species reactivityMouse
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:500 - 1:5,000 Fig 1
Western Blotting 1:1,000 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse MeCP2 (Cat. No. CS-106-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:136,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2
    Nuclear extracts of mouse fibroblasts (NH3T3, 40 μg) were analysed by Western blot using the Diagenode antibody against mouse MeCP2 (Cat. No. CS-106-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  •  実験手法
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  資料
    Datasheet MeCP2 CS-106-100 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  出版物

    How to properly cite this product in your work

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