|MagMeDIP-seq Package MANUAL|
Magnetic Methylated DNA Immunopreciptation Package for NGS. DNA methylation is a key epigenetic ...
Perform MeDIP (Methylated DNA Immunoprecipitation) followed by sequencing to get DNA methylation status of your sample using 5-methylcytosine antibody and magnetic beads. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.
This package contains everything you need from cDNA extraction to library preparation and quality check. It allows you to perform your NGS compatible MeDIP-seq assay, combining the strength of MagMeDIP technology with the power of our iDeal Library Preparation technique.
Figure 1. IP results obtained with Diagenode MagMeDIP qPCR Kit. MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.