Diagenode

MagMeDIP-seq Package

カタログ番号
フォーマット
価格
C02010040
10 rxns
$1,299.00

Perform MeDIP (Methylated DNA Immunoprecipitation) followed by sequencing to get DNA methylation status of your sample using 5-methylcytosine antibody and magnetic beads. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.

This package contains everything you need from cDNA extraction to library preparation and quality check. It allows you to perform your NGS compatible MeDIP-seq assay, combining the strength of MagMeDIP technology with the power of our iDeal Library Preparation technique.

Content

Characteristics

  • COMPLETE workflow from cDNA extraction to library preparation
  • Generate highly consistent results with internal controls in 24h
  • Minimize error with many reagents in 1 tube
  • Highest purification with IPure
  • Allows direct correlation between IP’d material & methylation status 

Figure 1. IP results obtained with Diagenode MagMeDIP qPCR Kit. MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.

  •  資料
    MagMeDIP-seq Package MANUAL
    Magnetic Methylated DNA Immunopreciptation Package for NGS. DNA methylation is a key epigenetic ...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MagMeDIP-seq Package (Diagenode Cat# C02010040). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    DNA methylation of the Tacr2 gene in a CUMS model of depression.
    Xiang D, Xiao J, Fu L, Yao L, Wan Q, Xiao L, Zhu F, Wang G, Liu Z
    Tacr2, the gene encoding the NK2 receptor, belongs to G protein-coupled receptors. Accumulating evidence has indicated that the tachykinin receptors may contribute to the pathophysiology of depression. During the last decade, some studies have shown that Tacr2 activation is involved in the modulation of emotional pr...

    Sensitive tumour detection and classification using plasma cell-free DNA methylomes.
    Shen SY, Singhania R, Fehringer G, Chakravarthy A, Roehrl MHA, Chadwick D, Zuzarte PC, Borgida A, Wang TT, Li T, Kis O, Zhao Z, Spreafico A, Medina TDS, Wang Y, Roulois D, Ettayebi I, Chen Z, Chow S, Murphy T, Arruda A, O'Kane GM, Liu J, Mansour M, McPher
    The use of liquid biopsies for cancer detection and management is rapidly gaining prominence. Current methods for the detection of circulating tumour DNA involve sequencing somatic mutations using cell-free DNA, but the sensitivity of these methods may be low among patients with early-stage cancer given the limited ...

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