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MagMeDIP-seq Package

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カタログ番号
フォーマット
C02010040
10 rxns
DISCONTINUED

MeDIP(メチル化DNA免疫沈降)の後にシーケンスを実行し、5-メチルシトシン抗体と磁気ビーズを使用してサンプルのDNAメチル化状態を検出します。 当社のキットには、最適化されたユーザーフレンドリーなプロトコールでメチル化DNAの最高濃縮を実現する、高品質の試薬が含まれています。

このパッケージには、cDNA抽出からライブラリー調製、品質チェックに及ぶ、必要な物が全て含まれています。 MagMeDIPテクノロジーの強さとiDeal Library調製テクニックのパワーを組み合わせることで、NGS互換のMeDIP-seqアッセイが可能です。

パッケージ内容

特徴

  • cDNA抽出からライブラリー調製までの完全なワークフロー
  • 24時間以内に内部統制で一貫性のある結果を生成
  • 1本のチューブに多数の試薬をまとめて、エラーを最小限に抑える
  • IPureによる最高の精製
  • IP化された材料とメチル化状態を直接相関可能

Figure 1. IP results obtained with Diagenode MagMeDIP qPCR Kit. MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.

  •  資料
    MagMeDIP-seq Package MANUAL
    Magnetic Methylated DNA Immunopreciptation Package for NGS. DNA methylation is a key epigenetic ...
    Download
    DNA Methylation Solutions BROCHURE
    Complete solution for DNA methylation studies! Whether you are experienced or new to th...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MagMeDIP-seq Package (Diagenode Cat# C02010040). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Polycystic ovary syndrome is transmitted via a transgenerational epigenetic process
    Mimouni et. al.
    Polycystic ovary syndrome (PCOS) is the most common reproductive and metabolic disorder affecting women of reproductive age. PCOS has a strong heritable component, but its pathogenesis has been unclear. Here, we performed RNA sequencing and genome-wide DNA methylation profiling of ...

    Integrated epigenetic biomarkers in circulating cell-free DNA as a robust classifier for pancreatic cancer.
    Cao F, Wei A, Hu X, He Y, Zhang J, Xia L, Tu K, Yuan J, Guo Z, Liu H, Xie D, Li A
    BACKGROUND: The high lethal rate of pancreatic cancer is partly due to a lack of efficient biomarkers for screening and early diagnosis. We attempted to develop effective and noninvasive methods using 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) markers from circulating cell-free DNA (cfDNA) for the det...

    Detection of renal cell carcinoma using plasma and urine cell-free DNA methylomes.
    Nuzzo PV, Berchuck JE, Korthauer K, Spisak S, Nassar AH, Abou Alaiwi S, Chakravarthy A, Shen SY, Bakouny Z, Boccardo F, Steinharter J, Bouchard G, Curran CR, Pan W, Baca SC, Seo JH, Lee GM, Michaelson MD, Chang SL, Waikar SS, Sonpavde G, Irizarry RA, Pome
    Improving early cancer detection has the potential to substantially reduce cancer-related mortality. Cell-free methylated DNA immunoprecipitation and high-throughput sequencing (cfMeDIP-seq) is a highly sensitive assay capable of detecting early-stage tumors. We report accurate classification of patients across all ...

    Detection and discrimination of intracranial tumors using plasma cell-free DNA methylomes.
    Nassiri F, Chakravarthy A, Feng S, Shen SY, Nejad R, Zuccato JA, Voisin MR, Patil V, Horbinski C, Aldape K, Zadeh G, De Carvalho DD
    Definitive diagnosis of intracranial tumors relies on tissue specimens obtained by invasive surgery. Noninvasive diagnostic approaches provide an opportunity to avoid surgery and mitigate unnecessary risk to patients. In the present study, we show that DNA-methylation profiles from plasma reveal highly specific sign...

    Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA.
    Shen SY, Burgener JM, Bratman SV, De Carvalho DD
    Circulating cell-free DNA (cfDNA) comprises small DNA fragments derived from normal and tumor tissue that are released into the bloodstream. Recently, methylation profiling of cfDNA as a liquid biopsy tool has been gaining prominence due to the presence of tissue-specific markers in cfDNA. We have previously reporte...

    DNA methylation of the Tacr2 gene in a CUMS model of depression.
    Xiang D, Xiao J, Fu L, Yao L, Wan Q, Xiao L, Zhu F, Wang G, Liu Z
    Tacr2, the gene encoding the NK2 receptor, belongs to G protein-coupled receptors. Accumulating evidence has indicated that the tachykinin receptors may contribute to the pathophysiology of depression. During the last decade, some studies have shown that Tacr2 activation is involved in the modulation of emotional pr...

    Sensitive tumour detection and classification using plasma cell-free DNA methylomes.
    Shen SY, Singhania R, Fehringer G, Chakravarthy A, Roehrl MHA, Chadwick D, Zuzarte PC, Borgida A, Wang TT, Li T, Kis O, Zhao Z, Spreafico A, Medina TDS, Wang Y, Roulois D, Ettayebi I, Chen Z, Chow S, Murphy T, Arruda A, O'Kane GM, Liu J, Mansour M, McPher
    The use of liquid biopsies for cancer detection and management is rapidly gaining prominence. Current methods for the detection of circulating tumour DNA involve sequencing somatic mutations using cell-free DNA, but the sensitivity of these methods may be low among patients with early-stage cancer given the limited ...

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イベント

  • DNA methylation workshop: Master MeDIP assay
    Diagenode
    Sep 30, 2021
  • DNA methylation workshop: Become an expert at RRBS
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    Oct 1-Oct 31, 2021
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