KDM6A polyclonal antibody - Classic

100 μl
  Bulk order

Polyclonal antibody raised in rabbit against KDM6A (lysine (K)-specific demethylase 6A), using a recombinant protein.

Concentration1.36 μg/μl
Species reactivityHuman, mouse
PurityPurified by peptide affinity chromatography.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 µg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 4
Immunohistochemistry 1:100 - 1:1,000 Fig 3, 6
Immunoprecipitation 1:100-1:500 Fig 5
Western Blotting 1:500 - 1:3,000 Fig 2

* Optimal dilutions/concentrations should be determined by the researcher.

  • Validation Data


    Figure 1. KDM6A ChIP results
    ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.

    Western Blot

    Figure 2. KDM6A WB results
    KDM6A antibody detects KDM6A protein by western blot analysis. A. 30 μg U87-MG whole cell extract B. 30 μg SK-N-SH whole cell extract C. 30 µg IMR32 whole cell extract D. 30 µg SK-N-AS whole cell extract 5 % SDS-PAGE KDM6A antibody dilution: 1:500


    Figure 3. KDM6A IHC results
    Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A antibody at a 1:250 dilution.


    Figure 4. KDM6A IFA results
    Immunofluorescence analysis of paraformaldehyde-fixed MCF-7 cells, using KDM6A antibody at a 1:500 dilution. 


    Figure 5. KDM6A IP results
    KDM6A antibody immunoprecipitates KDM6A protein in IP experiments. IP samples: SK-N-SH whole cell extract A. 30 μg SK-N-SH whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of KDM6A protein by 4 µg KDM6A antibody 5 % SDS-PAGE The immunoprecipitated KDM6A protein was detected by KDM6A antibody diluted 1:500. 


    Figure 6. KDM6A IHC results
    KDM6A antibody detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. KDM6A antibody dilution: 1:1,000. 

  •  実験手法
    Enzyme-linked immunosorbent assay. Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunohistochemistry Read more
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet KDM6A C15410237 DATASHEET
    Datasheet description
  •  出版物

    How to properly cite this product in your work

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