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Other names: VICKZ3, IMP-3, IMP3, KOC, KOC1, HKOC, CT98
Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C. |
| Storage Buffer | PBS containing 50% glycerol, does not contain a preservative. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| RIP | 15 µg per 107 cells | Fig 1 |
| Western blotting | 1:1,000 | Fig 2 |
| IP | 5 µg per 2.5x106 cells | Fig 3 |
Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3
Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3
Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3
Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (Cat. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP3 protein was subsequently detected by western blot with the IGF2BP3 antibody as described above.
| RIP RIP Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| IGF2BP3 polyclonal antibody datasheet DATASHEET Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Bin... | Download |
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Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2979', 'antibody_id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> VICKZ3, IMP-3, IMP3, KOC, KOC1, HKOC, CT98</p><p>Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3</strong><br />Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (<span>Cat</span>. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (<span>Cat</span>. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP3 protein was subsequently detected by western blot with the IGF2BP3 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410352', 'old_catalog_number' => '', 'sf_code' => 'C15410352-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 27, 2018', 'slug' => 'igf2bp3-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:45:46', 'created' => '2018-06-28 14:50:36', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => 'IGF2BP3 (UniProtKB/Swiss-Prot entry O00425) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the IGF2 and some other mRNA. It may repress translation of IGF2 during late development. Binding of IFG2BP3 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP also stabilizes the 3’ UTR of CD44 mRNA, promoting cell adhesion and invadopodia formation in cancer cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse: positive. 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Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'IGF2BP3 polyclonal antibody' $product = array( 'Product' => array( 'id' => '2979', 'antibody_id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> VICKZ3, IMP-3, IMP3, KOC, KOC1, HKOC, CT98</p><p>Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3</strong><br />Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (<span>Cat</span>. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (<span>Cat</span>. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). 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It binds to the 5’ UTR of the IGF2 and some other mRNA. It may repress translation of IGF2 during late development. Binding of IFG2BP3 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP also stabilizes the 3’ UTR of CD44 mRNA, promoting cell adhesion and invadopodia formation in cancer cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2979', 'antibody_id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> VICKZ3, IMP-3, IMP3, KOC, KOC1, HKOC, CT98</p><p>Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3</strong><br />Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (<span>Cat</span>. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (<span>Cat</span>. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP3 protein was subsequently detected by western blot with the IGF2BP3 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410352', 'old_catalog_number' => '', 'sf_code' => 'C15410352-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 27, 2018', 'slug' => 'igf2bp3-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:45:46', 'created' => '2018-06-28 14:50:36', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => 'IGF2BP3 (UniProtKB/Swiss-Prot entry O00425) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the IGF2 and some other mRNA. It may repress translation of IGF2 during late development. Binding of IFG2BP3 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP also stabilizes the 3’ UTR of CD44 mRNA, promoting cell adhesion and invadopodia formation in cancer cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3</strong><br />Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (<span>Cat</span>. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (<span>Cat</span>. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP3 protein was subsequently detected by western blot with the IGF2BP3 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410352', 'old_catalog_number' => '', 'sf_code' => 'C15410352-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 27, 2018', 'slug' => 'igf2bp3-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:45:46', 'created' => '2018-06-28 14:50:36', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => 'IGF2BP3 (UniProtKB/Swiss-Prot entry O00425) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the IGF2 and some other mRNA. It may repress translation of IGF2 during late development. Binding of IFG2BP3 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP also stabilizes the 3’ UTR of CD44 mRNA, promoting cell adhesion and invadopodia formation in cancer cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'Iigf2bp3-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:28:47', 'created' => '2018-06-27 15:28:47', 'select_label' => '535 - IGF2BP3 polyclonal antibody (001 - 1 μg/μl - Human, mouse: positive. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4927', 'product_id' => '2979', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'jpn' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '1016', 'name' => 'IGF2BP3 polyclonal antibody datasheet', 'description' => '<p>Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/igf2bp3-polyclonal-antibody-100-ug.pdf', 'slug' => 'igf2bp3-polyclonal-antibody-100-ug-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-11 15:55:29', 'created' => '2018-07-11 15:55:29', 'ProductsDocument' => array( 'id' => '2655', 'product_id' => '2979', 'document_id' => '1016' ) )include - 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2979', 'antibody_id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> VICKZ3, IMP-3, IMP3, KOC, KOC1, HKOC, CT98</p><p>Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3</strong><br />Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (<span>Cat</span>. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (<span>Cat</span>. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP3 protein was subsequently detected by western blot with the IGF2BP3 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410352', 'old_catalog_number' => '', 'sf_code' => 'C15410352-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 27, 2018', 'slug' => 'igf2bp3-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:45:46', 'created' => '2018-06-28 14:50:36', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => 'IGF2BP3 (UniProtKB/Swiss-Prot entry O00425) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the IGF2 and some other mRNA. It may repress translation of IGF2 during late development. Binding of IFG2BP3 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP also stabilizes the 3’ UTR of CD44 mRNA, promoting cell adhesion and invadopodia formation in cancer cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'IGF2BP3 polyclonal antibody' $product = array( 'Product' => array( 'id' => '2979', 'antibody_id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> VICKZ3, IMP-3, IMP3, KOC, KOC1, HKOC, CT98</p><p>Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3</strong><br />Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (<span>Cat</span>. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (<span>Cat</span>. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). 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It binds to the 5’ UTR of the IGF2 and some other mRNA. It may repress translation of IGF2 during late development. Binding of IFG2BP3 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP also stabilizes the 3’ UTR of CD44 mRNA, promoting cell adhesion and invadopodia formation in cancer cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'Iigf2bp3-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:28:47', 'created' => '2018-06-27 15:28:47', 'select_label' => '535 - IGF2BP3 polyclonal antibody (001 - 1 μg/μl - Human, mouse: positive. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4927', 'product_id' => '2979', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'jpn' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '1016', 'name' => 'IGF2BP3 polyclonal antibody datasheet', 'description' => '<p>Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/igf2bp3-polyclonal-antibody-100-ug.pdf', 'slug' => 'igf2bp3-polyclonal-antibody-100-ug-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-11 15:55:29', 'created' => '2018-07-11 15:55:29', 'ProductsDocument' => array( 'id' => '2655', 'product_id' => '2979', 'document_id' => '1016' ) )include - 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2979', 'antibody_id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> VICKZ3, IMP-3, IMP3, KOC, KOC1, HKOC, CT98</p><p>Polyclonal antibody raised in rabbit against human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3</strong><br />Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (<span>Cat</span>. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (<span>Cat</span>. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP3 protein was subsequently detected by western blot with the IGF2BP3 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410352', 'old_catalog_number' => '', 'sf_code' => 'C15410352-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '360', 'price_USD' => '360', 'price_GBP' => '320', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '900', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 27, 2018', 'slug' => 'igf2bp3-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP3 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:45:46', 'created' => '2018-06-28 14:50:36', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '535', 'name' => 'IGF2BP3 polyclonal antibody', 'description' => 'IGF2BP3 (UniProtKB/Swiss-Prot entry O00425) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the IGF2 and some other mRNA. It may repress translation of IGF2 during late development. Binding of IFG2BP3 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP also stabilizes the 3’ UTR of CD44 mRNA, promoting cell adhesion and invadopodia formation in cancer cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP3 (Cat. No. C15410352) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP3 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP3</strong><br />Whole cell extracts from K562, 293T, HeLa, Jurkat and NIH3T3 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP3 (<span>Cat</span>. No. C15410352) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410352-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP3</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP3 (<span>Cat</span>. No. C15410352, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). 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It binds to the 5’ UTR of the IGF2 and some other mRNA. It may repress translation of IGF2 during late development. Binding of IFG2BP3 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP also stabilizes the 3’ UTR of CD44 mRNA, promoting cell adhesion and invadopodia formation in cancer cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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