iDeal Library Preparation Kit x24 (incl. Index Primer Set 1)

24 rxns

The iDeal Library Preparation Kit reliably converts DNA into indexed libraries for next-generation sequencing, with input amounts down to 1 ng. Our kit offers a simple and fast workflow, high yields, and ready-to-sequence DNA on the Illumina platform. Combine this kit with our iDeal ChIP-seq kit for optimal ChIP-seq results. For library preparation of DNA inputs down to 50 pg, our MicroPlex Library Preparation Kit is an optimal choice.

  • 特徴
    • Validated with the IP-Star® Automated Platform
    • Multiplexing capacity of up to 24 samples
    • Only 3 hours from start to finish
    • Low GC bias with high fidelity amplification
    • Ideal for use with our optimized ChIP-seq kits

    High library yields

    High library yields
    5 ng of input DNA were used for each library prep. A. E. coli genomic DNA libraries. 1: Ladder; 2: Library prepared using iDeal Library Preparation™ Kit.
    B. FFPE sample. Human lung tumor genomic DNA 1: Library prepared using the iDeal Library Preparation™ Kit). 2: Input DNA (degraded FFPE genomic DNA).

    How it works

    iDeal Library preparation kit: how it works

  •  プロトコル集
    iDeal Library Preparation kit - IP-Star® compact
    The “iDeal Library Preparation” protocol on the IP-Star® is using the standard &l...
  •  実験手法
    主要な次世代シークエンシングプラットフォームのほとんどは、シークエンシングの前に 断片化したDNAまたはRNA に特異的なアダプターオリゴをライゲーションする必要があります。 入力DNAが断片化された後、それは末端修復され、平滑末端化されます。次のステップは、平滑化リン酸化DNAフラグメントの3 '末端にdAMPを加えて、コンカテマー化を防止し、相補的なdTオーバーハングを有するアダプターのライゲーションを可能にするA-テーリングです。さらに、増幅前または増幅中に多重化を容易にするた... Read more
    DNA断片化、ライブラリー調製、自動化:NGSのワンストップショップ 1. 断片化装置を選択してください:150 bp〜75 kbの範囲でDNAを断片化します。 5μlまで断片化:150 bp〜2 kbNGS DNAライブラリー調製およびFFPE核酸抽出に最適で、 2 kb〜75 kbの範囲をできます。メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで ... Read more
  •  資料
    iDeal Library Preparation kit x24 (incl. Index Primer Set 1) MANUAL
    The iDeal Library Preparation Kit reliably converts DNA into indexed libraries for next-g...
    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    iDeal ChIP-seq kit for Histones & Library Preparation kit MANUAL
    The iDeal ChIP-seq kit for Histones and the iDeal Library Preparation kit are the perfect combina...
  •   出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: iDeal Library Preparation Kit x24 (incl. Index Primer Set 1) (Diagenode Cat# C05010020). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Chromosome contacts in activated T cells identify autoimmune disease candidate genes
    Burren OS et al.
    BACKGROUND: Autoimmune disease-associated variants are preferentially found in regulatory regions in immune cells, particularly CD4+ T cells. Linking such regulatory regions to gene promoters in disease-relevant cell contexts facilitates identification of candidate disease genes. RESULTS: Within 4 h, act...

    DNA Replication Is Required for Circadian Clock Function by Regulating Rhythmic Nucleosome Composition
    Liu X. et al.
    Although the coupling between circadian and cell cycles allows circadian clocks to gate cell division and DNA replication in many organisms, circadian clocks were thought to function independently of cell cycle. Here, we show that DNA replication is required for circadian clock function in Neurospora. Genetic and ph...

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