Diagenode

iDeal ChIP-seq for Transcription Factors & MicroPlex Library Preparation™ Package

カタログ番号
フォーマット
価格
C01010056
10 ChIP and 12 library prep rxns
$1,610.00

DiagenodeのiDeal ChIP-seq Kit for Transcription Factorsは、安定した転写因子ChIP-seq結果をお届けする非常に特殊なソリューションです。 従来のソリューションとは異なり、当社のキットは高度に最適化されたプロトコールを採用し、幅広い種類の転写因子による検証が裏付けとなっています。 このキットは優れた特異性と感度で高収率を提供します。

  • 特徴
    • Confidence in results: Validated for ChIP-seq with multiple transcription factors
    • Proven: Validated by the epigenetics community, including the BLUEPRINT consortium
    • Most complete kit available for highest quality data - includes control antibodies and primers
    • Validated with Diagenode's MicroPlex Library Preparation™ kit and IP-Star® Automation

     

    ChIP-seq on cells

    CTCF Diagenode

    Figure 1. (A) Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade CTCF antibody. The IP'd DNA was subsequently analysed on an Illumina® HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in a region surrounding the GAPDH positive control gene.

    CTCF Diagenode

    Figure 1B. The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.

     

    ChIP-seq figure A

    ChIP-seq figure B

    ChIP-seq figure C

    Figure 2. Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade HDAC1 (A), LSD1 (B) and p53 antibody (C). The IP'd DNA was subsequently analysed on an Illumina® Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in regions of chromosome 3 (A), chromosome 12 (B) and chromosome 6 (C) respectively.

     

    ChIP-seq on tissue

    ChIP-seq figure A

    Figure 3A. Chromatin Immunoprecipitation has been performed using chromatin from mouse liver tissue, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade CTCF antibody. The IP'd DNA was subsequently analysed on an Illumina® HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in a region surrounding the Vwf positive control gene.

    Match of the Top40 peaks

    Figure 3B. The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.

  •  実験手法
    ChIP-seq
    検出方法(ChIP-seq)としてハイスループットシーケンシングと結合したクロマチン免疫沈降(ChIP)は、今やエピゲノム研究、すなわちゲノム規模でのタンパク質-DNA相互作用の研究において主要な方法の1つされています。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。 ChIP-seqワークフロー クロマチン調製 : DNAへのヒストンまたは転... Read more
  •  資料
    iDeal ChIP-seq Kit for Transcription Factors - Manual MANUAL
    Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution f...
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    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
    MicroPlex Library Preparation kit v2 Quick guide QUICK GUIDE
    MicroPlex Library Preparation v2 builds on the innovative MicroPlex chemistry to generate...
    Download
    MicroPlex Library Preparation kit v2 MANUAL
    MicroPlex v2 builds on the innovative MicroPlex chemistry to generate DNA libraries with ex...
    Download
  •  出版物

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