High Throughput 3’mRNA-seq Service

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ダイアジェノード社のエピゲノミクスプロファイリングサービスは、3’mRNA-seqライブラリー調製、マルチプレクシングシーケンス、およびデータ解析にぴったりな完全統一型のサービスワークフローを保証します。 このライブラリー調製は、初期のサンプルマルチプレックスプロトコールに基づいており、ポリアデニル化RNAの3 '末端に近い配列のイルミナ互換ライブラリーを生成します。


  • 信頼性の高い遺伝子発現の定量化
  • 遺伝子発現レベルの正確な比較
  • 多数サンプルの同時処理が可能
  • 非常に低品質のRNAサンプルにも対応
  • 迅速な納期(4〜6週間)



  • サンプル要件
    • Project size: minimum of 96 samples
    • Sample type: total isolated RNA
    • Optimal concentration: ≥ 20 ng/µL
    • Optimal volume: ≥10 µL
    • Shipment conditions: shipped in 96-well plates (low bind, RNase free, Eppendorf, 0030129504)
    • Batch effect: All the RNA samples intended to be used for the differential expression analysis should be included in the same library, bearing up to 96 samples
  • サービスワークフロー
    3mRNA-seq Service

    What our service includes

    1. RNA quality control (optional for additional fee)
      • Determination of RIN value (RNA integration number)
      • Measurement of RNA concentration
    2. Multiplexed library preparation
      • Reverse transcription
      • Sample multiplexing
      • Stranded library construction
      • Quality control of pooled libraries prior to sequencing
    3. Sequencing on Illumina platforms
      • 4 million raw reads per sample
    4. Data analysis (optional for additional fee)
      • Standard bioinformatic analysis
      • Advanced bioinformatic analysis
      • Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request
  • バイオインフォマティクス解析

    Standard bioinformatic analysis
    (optional for additional fee)

    Advanced bioinformatic analysis
    (optional for additional fee)

    • Demultiplexing, read quality control, trimming and filtering
    • Alignment to reference genome
    • Quantification of known transcripts

    Provided files:

    • Report with sequencing statistics
    • Raw data in FASTQ format
    • FastQC report
    • Alignment in BAM format
    • Un-normalized read counts
    • Differential expression analysis
    • Gene ontology terms analysis
    • Pathway analysis

  •  資料
    The Diagenode Epigenetics custom service POSTER
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    High-throughput 3’mRNA-seq FLYER
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: High Throughput 3’mRNA-seq Service (Diagenode Cat# G02140000). Click here to copy to clipboard.

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    Jul 4-Jul 6, 2022



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