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High Throughput 3’mRNA-seq Service

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カタログ番号
フォーマット
G02140000
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ハイスループット3’mRNA-seqは、Alithea Genomicsの独自の技術であるMercuriusを利用して、大規模な調査研究においても信頼性の高い遺伝子発現の定量化を可能にし、 多数のサンプルを用いて比較遺伝子発現解析を実行できる魅力的なソリューションです。また、関心のある複数の条件の評価や、より大きな複製セットを使用した場合の差次的発現解析の基礎となる統計的検出力の向上に最適です。

ダイアジェノード社のエピゲノミクスプロファイリングサービスは、3’mRNA-seqライブラリー調製、多重化シーケンス、およびデータ解析にぴったりな完全統一型のサービスワークフローを保証します。 このライブラリー調製は、初期のサンプルマルチプレックスを使用して、ポリアデニル化RNAの3 '末端に近い配列で、イルミナ互換ライブラリー調製をするMercuriusプロトコールに基づいています。

拡張性がり、手頃な3’mRNA-seq

  • 信頼性の高い遺伝子発現の定量化
  • 遺伝子発現レベルの正確な比較
  • 革新的なMercuriusテクノロジーを採用
  • 多数サンプルの同時処理が可能
  • 非常に低品質のRNAサンプルにも対応
  • 迅速な納期(4〜6週間)

初期のサンプル多重化を用いた革新的なアプローチ3mRNA-seq Service

その他のRNA-seqプロファイリングサービスはこちらから

  • サンプル要件
    • Project size: minimum of 96 samples
    • Sample type: total isolated RNA
    • Optimal concentration: ≥ 20 ng/µL
    • Optimal volume: ≥10 µL
    • Shipment conditions: shipped in 96-well plates (low bind, RNase free, Eppendorf, 0030129504)
    • Batch effect: All the RNA samples intended to be used for the differential expression analysis should be included in the same library, bearing up to 96 samples
  • サービスワークフロー
    3mRNA-seq Service

    What our service includes

    1. RNA quality control (optional for additional fee)
      • Determination of RIN value (RNA integration number)
      • Measurement of RNA concentration
    2. Multiplexed library preparation
      • Reverse transcription
      • Sample multiplexing
      • Stranded library construction
      • Quality control of pooled libraries prior to sequencing
    3. Sequencing on Illumina platforms
      • 4 million raw reads per sample
    4. Data analysis (optional for additional fee)
      • Standard bioinformatic analysis
      • Advanced bioinformatic analysis
      • Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request
  • バイオインフォマティクス解析

    Standard bioinformatic analysis
    (optional for additional fee)

    Advanced bioinformatic analysis
    (optional for additional fee)

    • Demultiplexing, read quality control, trimming and filtering
    • Alignment to reference genome
    • Quantification of known transcripts

    Provided files:

    • Report with sequencing statistics
    • Raw data in FASTQ format
    • FastQC report
    • Alignment in BAM format
    • Un-normalized read counts
    • Differential expression analysis
    • Gene ontology terms analysis
    • Pathway analysis

  •  資料
    The Diagenode Epigenetics custom service POSTER
    Complete workflows for genome-scale DNA methylation and histone marks analysis Epigenetics is cr...
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    Chromatin analysis DNA methylation services RNA-seq analysis
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    High-throughput 3’mRNA-seq FLYER
    High throughput 3’mRNA-seq is an attractive solution to perform comparative gene expression...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: High Throughput 3’mRNA-seq Service (Diagenode Cat# G02140000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing
    Daniel Alpern, Vincent Gardeux, Julie Russeil, Bastien Mangeat, Antonio C. A. Meireles-Filho, Romane Breysse, David Hacker and Bart Deplancke
    Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq ha...

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