Diagenode

HIF1 alpha polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15410234-100
100 μl
$315.00
  Bulk order

Polyclonal antibody raised in rabbit against HIF1 alpha (hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)), using a recombinant protein.

Lot42760
Concentration0.26 μg/μl
Species reactivityHuman, mouse, rat, cow: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 1% BSA, 20% Glycerol and 0.025% ProClin 300.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP 5 µg per ChIP Fig 1
Western blotting 1:1,000 Fig 2, 3
Immunoprecipitation 5 µg per IP Fig 4
Immunofluorescence 1:500 Fig 5
Immunohistochemistry 1:500 Fig 6

* Optimal dilutions/concentrations should be determined by the researcher.

  • Validation Data

    HIF1 alpha ChIP result

    Figure 1. ChIP results obtained with the Diagenode antibody directed against HIF1 alpha
    ChIP assays were performed using HepG2 cells and 5 µg of the Diagenode antibody against HIF1 alpha (Cat. No. C15410234). An equal amount of IgG was used as a negative IP control. QPCR was performed with primers specific for the VEGF promoter. Figure 1 shows the fold enrichment over the IgG negative control.

    A. HIF1 alpha WB result

    B. HIF1 alpha WB result


    Figure 2. Western blot analysis using the Diagenode antibody directed against HIF1 alpha
    Whole cell extracts (30 µg) from either HeLa (figure 2A) or NIH3T3 cells (figure 2B) were analysed by Western blot using the Diagenode antibody against HIF1 alpha (Cat. No. C15410234) diluted 1:1,000. Lane 2 represents extracts from cells treated with CoCl2 for 24 hours, lane 1 shows the result for untreated cells. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    HIF1 alpha WB result

    Figure 3. Western blot analysis using the Diagenode antibody directed against HIF1 alpha
    Whole cell extracts (30 µg) from MCF7 cells treated with CoCl2 for 24 hours were analysed by Western blot using the Diagenode antibody against HIF1 alpha (Cat. No. C15410234) diluted 1:1,000. Lane 1 shows the result of cells transfected with HIF1 alpha shRNA, whereas the result obtained with untransfected control cells is shown in lane 2. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    HIF1 alpha IP result

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against HIF1 alpha
    Immunoprecipitation was performed on whole cell extracts from HepG2 cells, treated with CoCl2 for 24 hours, using 5 µg of the Diagenode antibody against HIF1 alpha (Cat. No. C15410234, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated HIF1 alpha protein was detected by western blot with the HIF1 alpha antibody diluted 1:1,000.

    HIF1 alpha IF result

    Figure 5. Immunofluorescence with the Diagenode antibody directed against HIF1 alpha
    HeLa cells treated with CoCl2 for 24 hours (right) and untreated HeLa cells (left) were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against HIF1 alpha (Cat. C15410234) diluted 1:500 (green). The bottom panel shows costaining with phalloidin, a cytoskeleton marker (red).

    HIF1 alpha IHC result

    Figure 6. Immunohistochemistry using the Diagenode antibody directed against HIF1 alpha
    Formalin fixed paraffin embedded human kidney cancer cells were stained with the Diagenode antibody against HIF1 alpha (Cat. No. C15410234) diluted 1:500, followed by a peroxidase labelled goat anti-rabbit secondary antibody.

  •  実験手法
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet HIF1 alpha C15410234 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  出版物

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