Diagenode

HDAC3 monoclonal antibody - Classic

カタログ番号
フォーマット
価格
C15100145
(SN-145-100)
100 µl
$315.00
  Bulk order

Alternative names: HD3, RPD3, RPD3-2, SMAP45

Monoclonal antibody raised in mouse against human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.

Lot001
Concentrationnot determined
Species reactivityHuman
TypeMonoclonal
PurityConcentrated supernatant
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μl/ChIP Fig 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against HDAC3
    ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against HDAC3 (Cat. No. C15100145 ) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (Cat. No. C01010070), on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. Respectively, 5 μl of the HDAC3 monoclonal antibody and 5 μg of IgG (negative IP control) were used. QPCR was performed with primers for the promoters of the active genes c-fos (cat. No. pp-1004-050) and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  •  実験手法
  •  資料
    Datasheet HDAC3 SN-145-100 DATASHEET
    Datasheet description
    Download
  •  出版物

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