Diagenode

EZH2 polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15310039
(CS-039-100)
100 µl
DISCONTINUED

As an alternative we offer the purified EzH2 polyclonal antibody (C15410039)

Lot001
Concentrationnot determined
Species reactivityHuman, mouse
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA Not tested
Dot Blotting Not tested
Western Blotting 1:1,000 Fig 1
Gel Supershift Not tested
Immunofluorescence Not tested
Flow cytometry Not tested
Immunoprecipitation Not tested
ChIP 5 μl/ChIP Fig 2
  • Validation Data

    Western blot

    Figure 1 Western blot analysis using the Diagenode crude serum anti-EZH2.
    Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode crude serum directed against EZH2 (cat# CS-039-100) at dilution 1:1,000 in TBS-Tween + 5% skimmed milk. The antibody recognizes endogenous EZH2 in HeLa nuclear extract. On the right side, a molecular weight marker is shown (in kDa). The arrow indicates the location of the protein of interest.

    ChIP

    Figure 2. ChIP results obtained with the Diagenode purified antibody directed against EZH2.
    ChIP assays were performed using Jurkat cells (human peripheral blood leukemia T-cells), the polyclonal antibody directed against EZH2 (α Ezh2; Diagenode, cat# pAb-039-050) and the PCR primer set MYT1. IgG was used as negative control. Decreasing amounts of input were used for end-point PCR (lanes 1-3). The positive IP with the antibody anti-EZH2 gives a clear signal (lane 5), while the negative control gives no signal (lane 4). The figure shows the results for IgG purified antibody (2 μg/ChIP), however the crude serum (cat# CS-039-100) also works in ChIP at 5 μl/ChIP.

    Overview.
    Polycomb group (PcG) family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations [1,2]. EZH2 associates with the embryonic ectoderm development protein (Eed), the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems (NCBI GeneID: 2146: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=full_report&list_uids=2146). Over-expression of EZH2 is observed during advanced stages of prostate cancer [3] and breast cancer [4].

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet EZH2 CS-039-100 DATASHEET
    Datasheet description
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: EZH2 polyclonal antibody - Classic (Diagenode Cat# C15310039 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency.
    Pereira CF, Piccolo FM, Tsubouchi T, Sauer S, Ryan NK, Bruno L, Landeira D, Santos J, Banito A, Gil J, Koseki H, Merkenschlager M, Fisher AG
    Embryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG prot...

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