Diagenode

DNMT3A polyclonal antibody - Pioneer

カタログ番号
フォーマット
価格
C15310107
(CS-107-100)
100 µl
$210.00
  Bulk order

Alternative names: DNMT3A2, TBRS

Polyclonal antibody raised in rabbit against mouse DNMT3 (DNA methyltransferase 3A), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

LotA388-001
Concentrationnot determined
Species reactivityHuman, mouse
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:1,000 - 1:10,000 Fig 1
Western blotting 1:500 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against mouse DNMT3A (Cat. No. CS-107-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:86,500.

    Western blot

    Western blot analysis using the Diagenode antibody directed against DNMT3A
    Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against mouse DNMT3A (Cat. No. CS-107-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Target description

    DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.

  •  実験手法
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  資料
    Datasheet DNMT3A CS-107-100 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  出版物

    How to properly cite this product in your work

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    Oct 15-Oct 19, 2019
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    Oct 19-Oct 23, 2019
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