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Figure 1. Immunoprecipitation using the Diagenode antibody directed against CIRBP Immunoprecipitation was performed on total RNA isolated from 10 million Jurkat cells using 15 µg of the Diagenode antibody against CIRBP (Cat. No. C15410360) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the CIRBP antibody (upper right). The lower figure shows the gel image for the negative IgG control, the CIRBP antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. Western blot analysis using the Diagenode antibody directed against CIRBP Whole cell extracts from HeLa, 293T, K562, Jurkat and Rat1 cells (lanes 1 to 5, respectively) were analysed by Western blot using the Diagenode antibody against CIRBP (Cat. No. C15410360) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunoprecipitation using the Diagenode antibody directed against CIRBP Immunoprecipitation was performed on whole cell extracts from HeLa cells using 5 µg of the Diagenode antibody against CIRBP (Cat. No. C15410360, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CIRBP protein was subsequently detected by western blot with the CIRBP antibody as described above. The input is shown in lane 1.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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